계명대학교 의학도서관 Repository

Down-regulation of peroxisome proliferator-activated receptor gamma in human cervical carcinoma

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Author(s)
Tae-Il JungWon-Ki BaekSeong-Il SuhByeong-Churl JangDae-Kyu SongJae-Hoon BaeKun-Young KwonJi-Hyun BaeSoon-Do ChaInsoo BaeChi-Heum Cho
Keimyung Author(s)
Cha, Soon DoCho, Chi HeumBaek, Won KiSuh, Seong IlJang, Byeong ChurlSong, Dae KyuBae, Jae HoonKwon, Kun Young
Department
Dept. of Obstetrics & Gynecology (산부인과학)
Dept. of Microbiology (미생물학)
Dept. of Molecular Medicine (분자의학)
Dept. of Physiology (생리학)
Dept. of Pathology (병리학)
Journal Title
Gynecologic Oncology
Issued Date
2005
Volume
97
Issue
2
Abstract
Objective. Peroxisome proliferator-activated receptor gamma (PPARg) is a member of the nuclear hormone receptor superfamily.
Treatment of PPARg ligands has been shown to inhibit the growth of various human cancer cells. However, it has not been reported
whether human cervical carcinoma cells express PPARg. In this study, we investigated the expression of PPARg in human normal cervix
and cervical carcinoma tissues, and as well as the effect of PPARg ligands on cervical cancer cells survival.
Methods. Fresh cervical tissues from a study group of 10 study patients diagnosed with cervical carcinoma were analyzed for the
expression of PPARg using real-time RT-PCR and Western blot analysis. Immunohistochemical staining for PPARg was also performed
on the serial sections of 40 cervical carcinomas. In addition, we evaluated the feasibility of PPARg ligands, as a potential therapeutic drug
against cervical cancer cells using MTT assay and FACS analysis.
Results. We found that there were lower expression levels of PPARg mRNA and protein in cervical carcinoma tissues than in normal
cervical tissues. The extent and intensity of immunoreactive PPARg in normal cervix tissues were statistically much greater than those of
carcinoma tissues. In order to study effects of PPAR ligand on cell proliferation, we chose ciglitizone that showed very potent growth
inhibitory effects on the proliferation of two human cervical cancer cell lines (C-33-A and C-4II). C-4II cells express high expression of
PPARg, while C-33A cells express low level of PPARg. Treatment with ciglitizone inhibited the growth of C-4II cells in a dosedependent
manner, while the growth inhibitory effect of ciglitizone was much less in C-33A cells. In order to test whether ciglitizoneinduced
growth suppressive effects on cervical cancer cell lines is PPAR-dependent, we treated cervical cancer cells with ciglitizone and/
or GW9662 (a PPARg antagonist). No significant difference in cell survival was found in cells treated with ciglitizone alone vs. co-treated
with ciglitizone and GW9662. GW9662 alone did not induce any cell growth arrest in the cells that we used (data not shown). Thus, we
concluded that growth suppressive effects by ciglitizone may not be dependent upon status of PPAR expression. To clarify the mechanism
by which ciglitizone inhibits the growth of cervical carcinoma cells, flow cytometry and Western blotting assay were performed. As
results, we demonstrated that a large portion of C-4II cells (but not in C-33A) after ciglitizone treatment were arrest at G1 phase with the
induction of p21Cip1/Waf1 and p27kip1 protein.
Keimyung Author(s)(Kor)
차순도
조치흠
백원기
서성일
장병철
송대규
배재훈
권건영
Publisher
School of Medicine
Citation
Tae-Il Jung et al. (2005). Down-regulation of peroxisome proliferator-activated receptor gamma
in human cervical carcinoma. Gynecologic Oncology, 97(2), 365–373. doi: 10.1016/j.ygyno.2005.01.019
Type
Article
ISSN
0090-8258
Source
https://linkinghub.elsevier.com/retrieve/pii/S0090-8258(05)00044-2
DOI
10.1016/j.ygyno.2005.01.019
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35790
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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