계명대학교 의학도서관 Repository

LY294002 inhibits LPS-induced NO production through a inhibition of NF-кB activation: independent mechanism of phosphatidylinositol 3-kinase

Metadata Downloads
Author(s)
Young Ho KimKwang-Hae ChoiJong-Wook ParkTaeg Kyu Kwon
Keimyung Author(s)
Park, Jong WookKwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
Immunology letters
Issued Date
2005
Volume
99
Issue
1
Abstract
Phosphatidylinositol 3-kinase (PI3K) is critical player in cell proliferation and survival. The effects of LY294002 andwortmannin, inhibitors
of PI3K, on nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in lipoploysaccharide (LPS)-induced Raw264.7
cells were investigated. Significant inhibition of LPS-induced protein kinase B (PKB, Akt) phosphorylation occurred at 25 M LY294002 or
0.5 Mwortmannin. At the same concentrations, LY294002, but not wortmannin, significantly inhibited NO production and iNOS expression.
LY303511, an inactive analogue of LY294002, also inhibited NO production and iNOS expression. In addition, LY294002 and LY303511
significantly inhibited theDNAbinding activity of NF- Band NF- Bdependent reporter gene expression. These results suggest that LY294002
inhibits iNOS expression at least in part via inhibition of NF- B activation, independent of PI3K.
© 2004 Elsevier B.V. All rights reserved.
Keywords: Nitric oxide (NO); Inducible nitric oxide synthase (iNOS); LY294002; Phosphatidylinositol 3-kinase (PI3K); NF- B; Lipoploysaccharide
Keimyung Author(s)(Kor)
박종욱
권택규
Publisher
School of Medicine
Citation
Young Ho Kim et al. (2005). LY294002 inhibits LPS-induced NO production through a inhibition
of NF-кB activation: independent mechanism
of phosphatidylinositol 3-kinase. Immunology letters, 99(1), 45–50. doi: 10.1016/j.imlet.2004.12.007
Type
Article
ISSN
0165-2478
Source
https://www.sciencedirect.com/science/article/pii/S0165247804003736?via%3Dihub
DOI
10.1016/j.imlet.2004.12.007
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35856
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
공개 및 라이선스
  • 공개 구분공개
  • 엠바고Forever
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.