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Sulforaphane suppresses lipopolysaccharide-induced cyclooxygenase-2 (COX-2) expression through the modulation of multiple targets in COX-2 gene promoter

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Author(s)
Kyung Jin WooTaeg Kyu Kwon
Keimyung Author(s)
Kwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
International Immunopharmacology
Issued Date
2007
Volume
7
Issue
13
Abstract
Sulforaphane is a natural, biologically active compound extracted from cruciferous vegetables such as broccoli and cabbage. It
possesses potent anti-inflammation and anti-cancer properties. The mechanism by which sulforaphane suppresses COX-2 expression
remains poorly understood. In the present report, we investigated the effect of sulforaphane on the expression of COX-2 in
lipopolysaccharide (LPS)-activated Raw 264.7 cells. Sulforaphane significantly suppressed the LPS-induced COX-2 protein and
mRNA expression in a dose-dependent manner. The ability of sulforaphane to suppress the expression of the COX-2 was investigated
using luciferase reporters controlled by various cis-elements in COX-2 promoter region. Electrophoretic mobility shift assay (EMSA)
verified that NF-κB, C/EBP, CREB and AP-1 were identified as responsible for the sulforaphane-mediated COX-2 down-regulation.
In addition, we demonstrated the signal transduction pathway of mitogen-activated protein kinase (MAP kinase) in LPS-induced
COX-2 expression. Taken together, these results demonstrate that sulforaphane effectively suppressed the LPS-induced COX-2
protein via modulation of multiple core promoter elements (NF-κB, C/EBP, CREB and AP-1) in the COX-2 transcriptional regulation.
These results will provide new insights into the anti-inflammatory and anti-carcinogenic properties of sulforaphane.
© 2007 Elsevier B.V. All rights reserved.
Keimyung Author(s)(Kor)
권택규
Publisher
School of Medicine
Citation
Kyung Jin Woo and Taeg Kyu Kwon. (2007). Sulforaphane suppresses lipopolysaccharide-induced
cyclooxygenase-2 (COX-2) expression through the modulation of
multiple targets in COX-2 gene promoter. International Immunopharmacology, 7(13), 1776–1783. doi: 10.1016/j.intimp.2007.09.018
Type
Article
ISSN
1567-5769
Source
http://lps3.www.sciencedirect.com.proxy.dsmc.or.kr/science/article/pii/S1567576907003062?via%3Dihub
DOI
10.1016/j.intimp.2007.09.018
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35876
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
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