계명대학교 의학도서관 Repository

Age-related differential growth rate and response to 4-hydroxynonenal in mouse aortic smooth muscle cells

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Author(s)
TAE-JIN LEEJUNG-TAE LEESUNG-KWON MOONCHEORL-HO KIMJONG-WOOK PARKTAEG KYU KWON
Keimyung Author(s)
Park, Jong WookKwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Institute for Medical Science (의과학연구소)
Journal Title
International Journal of Molecular Medicine
Issued Date
2006
Volume
17
Issue
1
Abstract
. 4-Hydroxynonenal (4-HNE) is a peroxidation
product of ˆ-6-poly-unsaturated fatty acids and exerts growth
modifying as well as cytotoxic activities. This aldehyde
component of oxidized lipid is increased during the aging
process. In this study, to characterize the potential role of the
lipid peroxidation product in aging, we studied the effects of
4-HNE on cell proliferation and activation of cell-cycle
machinery and the mitogen-activated protein kinase signaling
pathway. 4-HNE-treated smooth muscle cells (SMCs) have
shown a different cell proliferation rate depending on 4-HNE's
incubation time and concentration. Interestingly, a prolonged
treatment of 0.1 μM 4-HNE (36 h) resulted in an increase of
cell growth in young SMCs but displayed cytotoxicity in
aged SMCs. Treatment with 4-HNE enhanced cyclin D1
expression and activation of the extracellular signal-regulated
kinase (ERK) signaling pathway, which were stronger in
young SMCs compared with aged SMCs. Moreover, 4-HNEinduced
cell proliferation and cyclin D1 expression were
significantly attenuated by PD98059, the ERK inhibitor, in
young SMCs. These data clearly indicate that increased cell
proliferation was associated with the induction of cyclin D1
expression which was regulated by ERK in 4-HNE-treated
young SMCs for 36 h. In contrast, we found that the cytotoxicity
of aged SMCs to 4-HNE was partly related to
generation of ROS and that pretreatment with N-acetyl-Lcysteine
prevented 4-HNE-induced cell death in aged SMCs.
These results suggest that the prolonged treatment of 0.1 μM
4-HNE-induced cell growth inhibition was caused by
generation of ROS. Collectively, the age-related different
growth rates and responses to 4-HNE are related to the
expression level of cyclin D1, activation of the ERK signaling
pathway, and regulation of ROS generation in SMCs.
Keimyung Author(s)(Kor)
박종욱
권택규
Publisher
School of Medicine
Citation
TAE-JIN LEE et al. (2006). Age-related differential growth rate and response to
4-hydroxynonenal in mouse aortic smooth muscle cells. International Journal of Molecular Medicine, 17(1), 29–35. doi: 10.3892/ijmm.17.1.29
Type
Article
ISSN
1107-3756
Source
https://www.spandidos-publications.com/ijmm/17/1/29
DOI
10.3892/ijmm.17.1.29
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35907
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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