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Marked transfection enhancement by the DPL (DNA/peptide/lipid) complex

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Author(s)
IK-JAE MOONHYUNGU KANGYOUNG-BAE SEUBYEONG-CHURL CHANGDAE-KYU SONGJONG-GU PARK
Keimyung Author(s)
Jang, Byeong ChurlPark, Jong GuSong, Dae Kyu
Department
Dept. of Molecular Medicine (분자의학)
Dept. of Physiology (생리학)
Journal Title
International Journal of Molecular Medicine
Issued Date
2007
Volume
20
Issue
4
Abstract
. A short peptide, corresponding to the nuclear
localization signal of the human immunodeficiency virus-1
Tat protein, Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg, was
modified by adding a cysteine residue at the COOH terminus.
The peptide was mixed with a reporter plasmid, and then
with cationic lipids, to form a tripartite complex, DNA/
peptide/lipid (DPL). Various cell lines were treated with the
DPL complex and compared for transfection efficiency with
those of the conventional DNA/lipid (DL) complex. With the
simple inclusion of the peptide, the DPL complex showed
much enhanced transfection. Meanwhile, the plasmid DNA
mixed only with the peptide exhibited some improvement but
with much lower transfection than the DPL complex. When
the DPL complex was formed with various cationic lipids,
the DOSPA/DOPE exhibited superior transfection efficiency
than the other cationic lipids tested at the optimal ratio of
1:3:5 (w:w:w) in many cell types. At the optimal ratio of the
DPL components, transfection efficiency was routinely
shown to be ~10-fold higher for reporter gene expression
than that of the conventional DL complex. Furthermore,
when subcutaneous tumors of a colon cancer cell line
(SW480) were treated intratumorally with antisense oligos,
k-ras-RiAS, delivered as a DPL complex, tumor growth was
markedly suppressed. This study shows that the DPL
complex, which is easy to formulate by ordered mixing, can
be employed for a much enhanced cellular uptake of a
transgene both in vitro and in vivo.
Keimyung Author(s)(Kor)
장병철
박종구
송대규
Publisher
School of Medicine
Citation
IK-JAE MOON et al. (2007). Marked transfection enhancement by the DPL
(DNA/peptide/lipid) complex. International Journal of Molecular Medicine, 20(4), 429–437. doi: 10.3892/ijmm.20.4.429
Type
Article
ISSN
1107-3756
Source
https://www.spandidos-publications.com/ijmm/20/4/429
DOI
10.3892/ijmm.20.4.429
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35913
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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