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Proteomic analysis of psoriatic skin tissue for identification of differentially expressed proteins: Up-regulation of GSTP1, SFN and PRDX2 in psoriatic skin

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Author(s)
JOOHYUN RYUSUNG GOO PARKBYOUNG CHUL PARKMISUN CHOEKYU-SUK LEEJAE-WE CHO
Keimyung Author(s)
Choe, Mi SunLee, Kyu SukCho, Jae We
Department
Dept. of Pathology (병리학)
Dept. of Dermatology (피부과학)
Journal Title
International Journal of Molecular Medicine
Issued Date
2011
Volume
28
Issue
5
Abstract
. Psoriasis is a chronic inflammatory skin disease,
characterized by a combination of abnormal proliferation
of keratinocytes, immunology and vascular proliferation.
Proteomic analyses have revealed some clues regarding the
pathogenesis of psoriasis. In the present study, we conducted
an investigation of different proteomes of psoriatic lesional
skin, and compared them with those of normal and nonlesional
psoriatic skin. We performed 2-D gel electrophoresis,
liquid chromatography tandem mass spectrometry (LC-MS/
MS) analysis and database searches. Expression of proteins
were evaluated by immunoblot and immunohistochemistry
analyses. Our data showed differential expression of 74 and
145 protein spots in non-lesional and lesional psoriatic skin,
respectively. Eleven of 36 proteins, which were identified
by LC-MS/MS, were categorized as apoptosis-regulating
proteins. Other protein spots were categorized as proteins
with involvement in the negative regulation of apoptosis,
defense response-related proteins and inflammatory response.
Of particular interest, increased expression of glutathione S
transferase 1 (GSTP1) and peroxiredoxin 2 (PRDX2), which
are involved in the Redox balance system, and SFN, which is
involved in the cellular proliferation system, was observed in
psoriatic lesional skin. Localization of GSTP1 and SFN was
observed above the middle layer of the epidermis in psoriatic
skin lesions. Expression of PRDX2 was clearly observed below
the middle layer of the epidermis in chronic type psoriatic skin
lesions. Taken together, 36 identified proteins were associated
with biological regulation, including regulation of cell death,
defense response, inflammatory response and reactive oxygen
species (ROS) regulation. PRDX2 and GSTP1 may play roles
in compensating mechanisms for reduction of ROS stress, and
SFN may play roles in prevention of cancer development in
proliferating cells through G2/M cell cycle arrest upon accidental
DNA damage within psoriatic skin lesions.
Keimyung Author(s)(Kor)
최미선
이규석
조재위
Publisher
School of Medicine
Citation
JOOHYUN RYU et al. (2011). Proteomic analysis of psoriatic skin tissue for identification
of differentially expressed proteins: Up-regulation
of GSTP1, SFN and PRDX2 in psoriatic skin. International Journal of Molecular Medicine, 28(5), 785–792. doi: 10.3892/ijmm.2011.757
Type
Article
ISSN
1107-3756
Source
https://www.spandidos-publications.com/ijmm/28/5/785
DOI
10.3892/ijmm.2011.757
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35919
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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