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Experimental pig model of clinically relevant wound healing delay by intrinsic factors

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Author(s)
Youngjin JungDaegu SonSunyoung KwonJunhyung KimKihwan Han
Keimyung Author(s)
Son, Dae GuKim, Jun HyungHan, Ki HwanKwon, Sun Young
Department
Dept. of Plastic Surgery (성형외과학)
Dept. of Pathology (병리학)
Journal Title
International Wound Journal
Issued Date
2013
Volume
10
Issue
3
Abstract
The intrinsic factors associated with delayed wounds are ischaemia, infection, the
presence of necrotic tissue and the presence of foreign bodies. This study was designed
to create adaptable intrinsic factors induced delayed wound healing model in the
pig, similar to clinical conditions. Four pigs were used in this study. Ten 4 × 4cm
fresh full-thickness skin defect wounds were made on the back of each pig. Double-
flanged blocks made of silicon, a material that induces foreign body reactions, tissue
ischaemia and causes local wound infection, were inserted into half of the wounds
(n = 5) and removed at weeks 1, 2 or 3. The other wounds (n = 5) were left open for
control purposes. Biopsies were obtained at weeks 1–4 for histological examinations
and to evaluate inflammatory cytokines, which included tumour necrosis factor-α
(TNF-α), interleukin (IL)-1β and IL-6. A Periscan PIM 3 Laser was used to measure
degrees of ischaemia. A Visitrak Digital

device was used to measure the sizes of
unhealed wounds. After 3 weeks, unhealed mean wound sizes were 30·72 ± 3·36
and 2·91 ± 1·51 cm
2
, in the experimental and control groups, respectively. Laser
Doppler showed limited perfusion at wound margins in wounds containing silicone
blocks. Histological findings corresponding to the severity of chronic inflammation
were markedly higher and persisted until week 3 in the experimental group. The
levels of IL-1 and TNF-α in the third week were statistically significantly higher in
the model group. Also, in the fourth week, the level of TNF-α in the model group
was significantly higher. Epithelialisation was not complete at the end of the fourth
week despite removing silicone blocks in the experimental group. On the basis of
macroscopic and histological evaluations and molecular assessments, the described
wound model has the characteristics of wound ischaemia and necrosis and persistent
inflammation, which are very similar to delayed wounds of patients
Keimyung Author(s)(Kor)
손대구
김준형
한기환
권선영
Publisher
School of Medicine
Citation
Youngjin Jung et al. (2013). Experimental pig model of clinically relevant wound healing
delay by intrinsic factors. International Wound Journal, 10(3), 295–305. doi: 10.1111/j.1742-481X.2012.00976.x
Type
Article
ISSN
1742-4801
Source
http://lps3.onlinelibrary.wiley.com.proxy.dsmc.or.kr/doi/abs/10.1111/j.1742-481X.2012.00976.x
DOI
10.1111/j.1742-481X.2012.00976.x
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35944
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. School of Medicine (의과대학) > Dept. of Plastic Surgery (성형외과학)
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