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Withaferin A inhibits tumor necrosis factor-α-induced expression of cell adhesion molecules by inactivation of Akt and NF-κB in human pulmonary epithelial cells

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Author(s)
Jung Hwa OhTaeg Kyu Kwon
Keimyung Author(s)
Kwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
International Immunopharmacology
Issued Date
2009
Volume
9
Issue
5
Abstract
We here investigated the functional effect of withaferin A on airway inflammation and its action mechanism.
Withaferin A inhibited the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell
adhesion molecule-1 (VCAM-1) in human lung epithelial A549 cells stimulated with tumor necrosis factor-α
(TNF-α), resulting in the suppression of leukocyte adhesion to lung epithelial A549 cells. In addition,
withaferin A inhibited TNF-α-induced expression of adhesion molecules (ICAM-1 and VCAM-1) protein
and mRNA in a dose-dependent manner. Withaferin A prevented DNA binding activity of nuclear factor-κB
(NF-κB) and nuclear translocation of NF-κB. It also inhibited phosphorylation of Akt and extracellular signalregulated
kinase (ERK), which are upstream in the regulation of adhesion molecules by TNF-α. Furthermore,
withaferin A inhibited U937 monocyte adhesion to A549 cells stimulated by TNF-α, suggesting that it may
inhibit the binding of these cells by regulating the expression of critical adhesion molecules by TNF-α. Taken
together, these results suggest that withaferin A inhibits cell adhesion through inhibition of ICAM-1 and
VCAM-1 expression, at least in part, by blocking Akt and down-regulating NF-κB activity.
Keimyung Author(s)(Kor)
권택규
Publisher
School of Medicine
Citation
Jung Hwa Oh and Taeg Kyu Kwon. (2009). Withaferin A inhibits tumor necrosis factor-α-induced expression of cell adhesion
molecules by inactivation of Akt and NF-κB in human pulmonary epithelial cells. International Immunopharmacology, 9(5), 614–619. doi: 10.1016/j.intimp.2009.02.002
Type
Article
ISSN
1567-5769
Source
http://lps3.www.sciencedirect.com.proxy.dsmc.or.kr/science/article/pii/S1567576909000630?via%3Dihub
DOI
10.1016/j.intimp.2009.02.002
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35959
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
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