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Prevention of CCl4-induced liver cirrhosis by ribbon antisense to transforming growth factor-ß1

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Author(s)
KYUNG-OH DOHHYUN-KYUNG JUNGIK-JAE MOONHYUN-GU KANGJEONG-HOH PARKJONG-GU PARK
Keimyung Author(s)
Park, Jong Gu
Department
Dept. of Molecular Medicine (분자의학)
Institute for Medical Science (의과학연구소)
Journal Title
International Journal of Molecular Medicine
Issued Date
2008
Volume
21
Issue
1
Abstract
. Transforming growth factor-ß1 (TGF-ß1) is an
important mediator of tissue fibrosis, including liver cirrhosis.
Ribbon-type antisense oligonucleotide to TGF-ß1 (TGF-ß1
RiAS) was designed and combined with cationic peptide
derived from the nuclear localization signal of human
immunodeficiency virus-1 Tat protein for enhanced cellular
uptake. When Hepa1c1c7 cells were transfected with TGF-ß1
RiAS, the level of TGF-ß1 mRNA was reduced by >70%.
TGF-ß1 RiAS, mismatched RiAS, and normal saline were
each injected into mice via the tail vein, beginning the week
after intraperitoneal CCl4 injection and continuing for 7
weeks, in order to determine whether TGF-ß1 RiAS prevents
the fibrotic changes induced by the CCl4 injection. After 8
weeks of the experiment, all of the mice treated with TGF-ß1
RiAS survived, compared to 50% of the control group and
65% of the mismatched RiAS-treated group. Upon
examining the biochemical effects on the liver, TGF-ß1
mRNA levels were reduced significantly only in the TGF-ß1
RiAS-treated group. Immunohistochemical studies showed a
reduced accumulation of collagen and α-smooth muscle
actin. Our experimental results suggest that ribbon antisense
to TGF-ß1, with efficient uptake, effectively blocks the
expression of TGF-ß1 and prevents fibrosis of the liver
Keimyung Author(s)(Kor)
박종구
Publisher
School of Medicine
Citation
KYUNG-OH DOH et al. (2008). Prevention of CCl4-induced liver cirrhosis by
ribbon antisense to transforming growth factor-ß1. International Journal of Molecular Medicine, 21(1), 33–39. doi: 10.3892/ijmm.21.1.33
Type
Article
ISSN
1107-3756
Source
https://www.spandidos-publications.com/ijmm/21/1/33
DOI
10.3892/ijmm.21.1.33
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35989
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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