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Withaferin A induces apoptosis through the generation of thiol oxidation in human head and neck cancer cells

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Author(s)
JONG WON PARKKYOUNG-JIN MINDONG EUN KIMTAEG KYU KWON
Keimyung Author(s)
Kim, Dong EunKwon, Taeg Kyu
Department
Dept. of Otorhinolaryngology (이비인후과학)
Dept. of Immunology (면역학)
Journal Title
International Journal of Molecular Medicine
Issued Date
2015
Volume
35
Issue
1
Abstract
. Withaferin A is a steroidal lactone purified from the Indian medicinal plant, Withania somnifera. Withaferin A has been shown to inhibit the proliferation, metastasis, invasion and angiogenesis of cancer cells. In the present study, we investigated whether withaferin A induces apoptosis in the human head and neck cancer cells, AMC-HN4. Withaferin A markedly increased the sub-G1 cell population and the cleavage of poly(ADP-ribose) polymerase (PARP), which are markers of apoptosis. Pan-caspase inhibitor, z-VAD-fmk (z-VAD), markedly inhibited the withaferin A-induced apoptosis. However, the withaferin A-induced increase in the expression of COX-2 was not affected by treatment with z-VAD. Furthermore, withaferin A upregulated cyclooxygenase-2 (COX-2) expression. The COX-2 inhibitor, NS-398, reduced the withaferin A-induced production of prostaglandin E2. However, treatment with NS-398 did not affect the sub-G1 population and the cleavage of PARP. In addition, the withaferin A-induced apoptosis was independent of reactive oxygen species production. Thiol donors [N-acetylcysteine (NAC) and dithiothreitol (DTT)] reversed withaferin A-induced apoptosis. Therefore, our data suggest that withaferin A induces apoptosis through the mechanism of thiol oxidation in head and neck carcinoma cells.
Keimyung Author(s)(Kor)
김동은
권택규
Publisher
School of Medicine
Citation
JONG WON PARK et al. (2015). Withaferin A induces apoptosis through the generation of thiol oxidation in human head and neck cancer cells. International Journal of Molecular Medicine, 35(1), 247–252. doi: 10.3892/ijmm.2014.1983
Type
Article
ISSN
1107-3756
Source
https://www.spandidos-publications.com/ijmm/35/1/247
DOI
10.3892/ijmm.2014.1983
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35994
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학)
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