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The comparative immunotoxicity of mesoporous silica nanoparticles and colloidal silica nanoparticles in mice

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Author(s)
Soyoung LeeMi-Sun KimDakeun LeeTaeg Kyu KwonDongwoo KhangHui-Suk YunSang-Hyun Kim
Keimyung Author(s)
Kwon, Taeg Kyu
Department
Dept. of Immunology (면역학)
Journal Title
International Journal of Nanomedicine
Issued Date
2013
Volume
8
Issue
1
Abstract
Background: Mesoporous silica (MPS) nanoparticles (NPs), which have a unique pore structure
and extremely large surface area and pore volume, have received much attention because
of their biomedical application potential. Using MPS NPs for biomedical devices requires the
verification of their biocompatibility because the surface area of NPs is one of the most important
determinants of toxicity, including the cellular uptake and immune response. We have previously
reported that the cytotoxicity and inflammation potential of MPS NPs have been shown
to be lower than those of general amorphous colloidal silica (Col) NPs in macrophages, but the
low cytotoxicity does not guarantee high biocompatibility in vivo. In this study, we compared
the in vivo immunotoxicity of MPS and Col NPs in the mouse model to define the effects of
pore structural conditions of silica NPs.
Materials and methods: Both MPS and Col NPs (2, 20, and 50 mg/kg/day) were intraperitoneally
administered in female BALB/c mice for 4 weeks, and clinical toxicity, lymphocyte
population, serum IgG/IgM levels, and histological changes were examined.
Results: There was no overt sign of clinical toxicity in either MPS- or Col-treated mice.
However,
MPS NPs led to significant increases in liver and spleen weight and splenocyte
proliferation.
Mice treated with MPS NPs showed altered lymphocyte populations (CD3+,
CD45+, CD4+, and CD8+) in the spleen, increased serum IgG and IgM levels, and histological
changes. Despite slight changes in lymphocyte populations in the spleen, Col NPs did not alter
other immunological factors.
Conclusion: The results indicate that in vivo exposure to MPS NPs caused more damage to
systemic immunity than that of Col NPs through the dysregulation of the spleen. The results
for in vivo data are inconsistent with those for in vitro data, which show lower cytotoxicity for
MPS NPs. These results suggest the importance of verifying biocompatibility both in vitro and
in vivo during the design of new nanomaterials.
Keywords: immunotoxicity, mesoporous silica nanoparticle, colloidal silica nanoparticle,
spleen
Keimyung Author(s)(Kor)
권택규
Publisher
School of Medicine
Citation
Soyoung Lee et al. (2013). The comparative immunotoxicity of mesoporous
silica nanoparticles and colloidal silica
nanoparticles in mice. International Journal of Nanomedicine, 8(1), 147–158. doi: 10.2147/IJN.S39534
Type
Article
ISSN
1176-9114
DOI
10.2147/IJN.S39534
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/35997
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
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