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Increased Expression of ATP-sensitive K+ Channels Improves the Right Ventricular Tolerance to Hypoxia in Rabbit Hearts

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Author(s)
Hyoung Kyu KimSeong Woo ChoiJun Seok AhnNari KimTae-Hoon ChoiSung-Woo ParkEn A KoWon Sun ParkDae-Kyu SongJin Han
Keimyung Author(s)
Song, Dae Kyu
Department
Dept. of Physiology (생리학)
Journal Title
Korean J of Physiology & Pharmacology
Issued Date
2011
Volume
15
Issue
4
Keyword
ATP-sensitive K+ channelsKir6.2Right ventricleHypoxia
Abstract
ATP-sensitive K+ channels (KATP) are major component of preventing ischemia-reperfusion injury.
However, there is little information regarding to the expressional difference of KATP and its function
between left and right ventricles. In this study, we measured the lactate dehydrogenase release of
rabbit heart slices in vitro and determined the difference of the KATP expression at the both ventricles
by measuring the level of KATP-forming Kir6.2 (OcKir6.2) mRNA using in situ hybridization. The hearts
were preconditioned with 15 min hypoxia and reoxygenated for 15 min before a hypoxic period of 60
min, followed by reoxygenation for 180 min. With hypoxic preconditioning (100% N2) with 15 min,
left ventricles (LV) showed higher release of LDH comparing with right ventricles (RV). Adding KATP
blocker glibenclamide (10μM) prior to a hypoxic period of 60 min, hypoxic preconditioning effect of
RV was more abolished than LV. With in situ hybridization, the optical density of OcKir6.2 was higher
in RV. Therefore, we suggest that different KATP expression between LV and RV is responsible for
the different response to hypoxia and hypoxic preconditioning of rabbit hearts.
Keimyung Author(s)(Kor)
송대규
Publisher
School of Medicine
Citation
Hyoung Kyu Kim et al. (2011). Increased Expression of ATP-sensitive K+ Channels Improves the Right Ventricular Tolerance to Hypoxia in Rabbit Hearts. Korean J of Physiology & Pharmacology, 15(4), 189–194. doi: 10.4196/kjpp.2011.15.4.189
Type
Article
ISSN
1226-4512
DOI
10.4196/kjpp.2011.15.4.189
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/36110
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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