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Alpha-lipoic acid attenuates methionine choline deficient diet-induced steatohepatitis in C57BL/6 mice

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Author(s)
Ae-Kyung MinMi-Kyung KimHye-Soon KimHye-Young SeoKi-Up LeeJung-Guk KimKeun-gyu ParkIn-Kyu Lee
Keimyung Author(s)
Kim, Mi KyungKim, Hye Soon
Department
Dept. of Internal Medicine (내과학)
Journal Title
Life Science
Issued Date
2012
Volume
90
Issue
Issues 5-6
Keyword
Alpha-lipoic acidCYP2E1ER stressMAP kinaseSteatohepatitis
Abstract
Aims: Non-alcoholic steatohepatitis (NASH) is a liver disease that causes fat accumulation, inflammation and fibrosis. Increased oxidative stress contributes to hepatic inflammation and fibrosis by upregulation of Cytochrome P450 2E1 (CYP2E1), endoplasmic reticulum (ER) stress andmitogen-activated protein kinase (MAPK) activity. This study examined whether alpha-lipoic acid (ALA), a naturally occurring thiol antioxidant, prevents steatohepatitis through the inhibition of several pathways involved in hepatic inflammation and fibrosis. Main Methods: C57BL/6 mice were fed an MCD diet with or without ALA for 4 weeks. Liver sections from mice on control or MCD diets with or without ALA were stained with hematoxylin-eosin, oil red O, and anti-4-HNE antibody. The effects of ALA on methionine-choline deficient MCD-diet induced plasma AST and ALT as well as tissue TBARSweremeasured. The effects ofALAonCYP2E1 expression, ER stress,MAPK levels, and NF-κB activity inMCD diet-fed mice liver were measured by northern and western blot analysis. Key findings: Dietary supplementation with ALA reducedMCD diet-induced hepatic lipid accumulation, hepatic inflammation, TBARS, 4-HNE, and plasma ALT and AST levels. These effects were associated with a reduced expression of CYP2E1 and reduced ER stress and MAPK and NF-κB activity. Significance: Taken together, the results of the present study indicate that ALA attenuates steatohepatitis through inhibition of several pathways, and provide the possibility that ALA can be used to prevent the development and progression of non-alcoholic fatty liver disease in patients who have strong risk factors for NASH.
Keimyung Author(s)(Kor)
김미경
김혜순
Publisher
School of Medicine
Citation
Ae-Kyung Min et al. (2012). Alpha-lipoic acid attenuates methionine choline deficient diet-induced steatohepatitis in C57BL/6 mice. Life Science, 90(Issues 5-6), 200–205. doi: 10.1016/j.lfs.2011.11.012
Type
Article
ISSN
0024-3205
Source
https://www.sciencedirect.com/science/article/pii/S0024320511005741?via%3Dihub
DOI
10.1016/j.lfs.2011.11.012
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/36299
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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