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Inhibitory Effect of Farfarae Flos Water Extract on COX-2, iNOS Expression and Nitric Oxide Production in lipopolysaccharide - activated RAW 264.7 cells

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Author(s)
Tae Gyoung YoonBoo Hyeong ByunTeag Kyu KwonSeong ll SuhSung HuI ByunYoung Kyu KwonSang Chan Kim
Keimyung Author(s)
Kwon, Taeg KyuSuh, Seong Il
Department
Dept. of Immunology (면역학)
Dept. of Microbiology (미생물학)
Journal Title
동의생리병리학회지
Issued Date
2004
Volume
18
Issue
3
Keyword
Farfrae FlosiNOSCOX-2TNF-αIL-1βIL-6
Abstract
Farfrae Flos has been clinically used for the treatment of asthma in traditional oriental medicine. There is lack of studies regarding the effects of Farfrae Flos on the immunological activities. The present study was conducted to evaluate the effect of Farfrae Flos on the regulatory mechanism of cytokines and nitric oxide (NO) for the immunological activities in Raw 264.7 cells. In Raw 264.7 cells stimulated with lipopolysaccharide (LPS) to mimic inflammation, Farfrae Flos water extract inhibited nitric oxide production in a dose-dependent manner and abrogated inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2). Farfrae Flos water extract did not affect on cell viability. To investigate the mechanism by which Farfrae Flos water extract inhibits iNOS and COX-2 gene expression, we examined the on the phospholylation of inhibitor κBα and production of TNF-α, IL-1β and IL-6. Results provided evidence that Farfrae Flos inhibited the production of interleukin-1β (IL-1β) and the activation of phospholylation of inhibitor κBα in Raw 264.7 cells activated with LPS. These findings suggest that Farfrae Flos can produce anti-inflammatory effect, which may play a role in adjunctive therapy in Gram-negative bacterial infections.
Keimyung Author(s)(Kor)
권택규
서성일
Publisher
School of Medicine
Citation
Tae Gyoung Yoon et al. (2004). Inhibitory Effect of Farfarae Flos Water Extract on COX-2, iNOS Expression and Nitric Oxide Production in lipopolysaccharide - activated RAW 264.7 cells. 동의생리병리학회지, 18(3), 908–913.
Type
Article
ISSN
1738-7698
Source
http://www.dbpia.co.kr/Journal/ArticleDetail/NODE02389170
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/36468
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
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