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桑黃 물추출물이 LPS로 유도된 Raw 264.7 cell에서의 TNF-α, IL-1β, IL-6 및 Nitric Oxide production에 미치는 영향

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Author(s)
김상찬정연숙이재령김영우변부형권택규서성일변성희권영규
Keimyung Author(s)
Kwon, Taeg KyuSuh, Seong Il
Department
Dept. of Immunology (면역학)
Dept. of Microbiology (미생물학)
Journal Title
동의생리병리학회지
Issued Date
2004
Volume
18
Issue
3
Keyword
TNF-αIL-1β
Abstract
Phellinus igniarius has been clinically used for the treatment of hemorrhoidal fistula, dysmenorrhea and the
prevention of cancer in traditional oriental medicine. Recent studies showed that Phellinus igniarius produced
anti-cancer, anti-metastasis and immuno-modulatory effects. There is lack of studies regarding the effects of Phellinus
igniarius on the immunological activities. The present study was conducted to evaluate the effect of Phellinus igniarius
on the regulatory mechanism of cytokines and nitric oxide (NO) for the immunological activities in Raw 264.7 cells.
After the treatment of Phellinus igniarius water extract, cell viability was measured by MTT assay, NO production was
monitored by measuring the nitrite content in culture medium. COX-2 and iNOS were determined by Immunoblot
analysis, and levels of cytokine were analyzed by sandwich immunoassays. Results provided evidence that Phellinus
igniarius inhibited the production of nitrite and nitrate (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β)
and interleukin-6 (IL-6), and the activation of phospholylation of inhibitor κBα (p-IκBα) in Raw 264.7 cells activated with
lipopolysaccharide (LPS). These findings suggest that Phellinus igniarius can produce anti-inflammatory effect, which
may play a role in adjunctive therapy in Gram-negative bacterial infections.
Alternative Title
Inhibitory Effect of Phellinus Igniarius water extract on TNF-α, IL-1β, IL-6 and Nitric Oxide Production in lipopolysaccharide - activated Raw 264.7 cells
Keimyung Author(s)(Kor)
권택규
서성일
Publisher
School of Medicine
Citation
김상찬 et al. (2004). 桑黃 물추출물이 LPS로 유도된 Raw 264.7 cell에서의 TNF-α, IL-1β, IL-6 및 Nitric Oxide production에 미치는 영향. 동의생리병리학회지, 18(3), 880–886.
Type
Article
ISSN
1738-7698
Source
http://www.dbpia.co.kr/Journal/ArticleDetail/NODE02389166
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/36470
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
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