계명대학교 의학도서관 Repository

Cisplatin에 의한 뇌세포사멸에서 補中免疫丹의 방어효과

Metadata Downloads
Author(s)
유경태문석재원진희김동웅이종덕원경숙문구
Keimyung Author(s)
Won, Kyoung Sook
Department
Dept. of Nuclear Medicine (핵의학)
Journal Title
동의생리병리학회지
Issued Date
2003
Volume
17
Issue
2
Keyword
Bojungmyunyuk-dan(補中免疫丹)CisplatinAstrocyteApoptotic cell deathCaspase protease family
Abstract
This study was designed to investigate the protective effect of Bojungmyunyuk-dan(BJMY-Dan) on the cisplatin-induced cytotoxicity of primary rat astrocytes. BJMY-Dan is an oriental herbal prescription for its ability to recover protectve effects against anti-cancer chemotherapies. After astrocytes were treated cisplafin, MTT assay was performed for cell viability test. To explore the mechanism of cytotoxicity, I used the several measures of apoptosis to determine whether this processes was involved in cisplatin-induced cell damage in astrocytes. AJso, astrocytes were treated with BJMY-Dan and then, followed by the addition of cisplatin. Cisplatin decreased the viability of astrocytes in a dose and time-dependent manner. BJMY-Dan increased the viability of astrocytes treated cisplatin. Astrocytes treated cisplatin were revealed as apoptosis characterized by nuclear staining and flow cytometry. BJMY-Dan protected astrocytes from cisplatin-induced nuclear fragmentation and chromatin condensation. Also, caspase-3 and caspase-9 proteases were activated in astrocytes by cisplatin. BJMY-Dan inhibited the activation of caspase proteases in cisplatin-treated astrocytes.
Cleavage of [poly(ADP-ribose) polymerase](PARP) was occurred at 12hr after treatment of cisplatin in astrocytes. BJMY-Dan recovered the cleavage of PARP in cisplatin-treated astrocytes. Also, BJMY-Dan inhibited the activation of pro-apoptotic factor, Bak by cisplatin. Lastly, astrocytes stained with JC-1 and Rhodamine 123 were photographed by fluorescence microscope to visualize changes of mitochondrial membrane permeability transition(MPT) during treatment with cisplatin for 24hr. BJMY-Dan recovered the change of MPT by cisplatn in astrocytes. According to above results, BJMY-Dan may protect astrocytes from cytotoxicity induced by chemotherapeutic agents, including cisplatin.
Alternative Title
Protectivw Effects of Bojungmyunyuk-dan in Cisplatin Treated Brain Cell Death
Keimyung Author(s)(Kor)
원경숙
Publisher
School of Medicine
Citation
유경태 et al. (2003). Cisplatin에 의한 뇌세포사멸에서 補中免疫丹의 방어효과. 동의생리병리학회지, 17(2), 394–402.
Type
Article
ISSN
1738-7698
Source
http://www.dbpia.co.kr/Journal/ArticleDetail/NODE02398149
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/36474
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Nuclear Medicine (핵의학)
공개 및 라이선스
  • 공개 구분공개
  • 엠바고Forever
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.