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대뇌 기저핵 신경세포에서 Nitric Oxide를 매개로 한 망간의 세포독성

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Author(s)
신동훈정용욱배재훈송대규박원균고복현김두희
Keimyung Author(s)
Shin, Dong HoonPark, Won KyunBae, Jae HoonSong, Dae Kyu
Department
Dept. of Preventive Medicine (예방의학)
Dept. of Medical Education (의학교육학)
Dept. of Physiology (생리학)
Journal Title
예방의학회지
Issued Date
1999
Volume
32
Issue
4
Keyword
Manganess(Mn)Ntric oxide(NO)Nitric oxide synthase(NOS)NeurotoxicityNeurons
Abstract
Objectives:eurotoxicity is mediated by nitric oxide(NO) in the rat primary neuronal cultures and assess the effect of Mn 2+ Mn2+ on the N-methyl-D aspartate(NMDA) receptors. Methods: We have used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)assay to examine the effect of cytotoxicity of MnCl 2 MnCl2 in neuronal cells , NO production was determined by measuring nirites, a stable oxidation product of NO. The neurons in the rat that contains neuronal nitric oxide synthase(nNOS) were examined by immunofluorescence and confocal microscopy. The effects of Mn 2+ Mn2+ on the NMDA receptors was assesed by the whole cell voltage clamp technique. Results: We showed that the NO release and NOS expression was increased with 500uM MnCl 2 MnCl2 treatment and an NOS inhibitors, N G −nitro−L−arginine NG−nitro−L−arginine , prevented neurotoxicity elicited by manganese. In the electrophysiological study, Mn 2+ Mn2+ does not block or activate the NMDA receptors and not pass through the NMDA receptors in a neurons of basal ganglia. Conclusions: It is concluded that manganese neurotoxicity in basal ganglia was partially mediated by nitric oxide in the cell culture model.
이상의 결론을 근거로 다음과 같은 제언을 하고자 한다.
Alternative Title
Nitric Oxide-Mediated Cytotoxicity of Manganese in Basal Ganglia Neuronal Cell
Keimyung Author(s)(Kor)
신동훈
박원균
배재훈
송대규
Publisher
School of Medicine
Citation
신동훈 et al. (1999). 대뇌 기저핵 신경세포에서 Nitric Oxide를 매개로 한 망간의 세포독성. 예방의학회지, 32(4), 459–466.
Type
Article
ISSN
0254-5985
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/36821
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Medical Education (의학교육학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
1. School of Medicine (의과대학) > Dept. of Preventive Medicine (예방의학)
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