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Hypoglycemic effects of submerged culture of Ceriporia lacerata mycelium

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Author(s)
Eun Ji ShinJi-Eun KimJi-Hye KimYong Man ParkSung Kyoon YoonByeong-Churl JangSam-Pin LeeByoung-Cheon Kim
Keimyung Author(s)
Jang, Byeong Churl
Department
Dept. of Molecular Medicine (분자의학)
Journal Title
한국식품저장유통학회지
Issued Date
2015
Volume
22
Issue
1
Keyword
Ceriporia laceratadiabeteshypoglycemic effect
Abstract
To investigate the hypoglycemic effect of the submerged culture of the Ceriporia lacerata mycelium (CL01) species, in-vitro and in-vivo tests were executed using INS-1 and 3T3-L1 cells, normal and diabetic mice. CL01 exhibited an inhibitory effect on cell death through dexamethasone in the INS-1 cells, and increased the GLUT4 expression in the 3T3-L1 cells. A hematological monitoring test was executed using diabetic mice divided into four groups : normal control (G1), negative control (G2), positive control (G3), and CL01 250 mg/kg (G4) groups, which were fed daily for 6 weeks. The body weight gain, food intake, and water intake of G4 were not significantly different from those of G2. After 5 weeks, the blood glucose levels of G4 were significantly different from those of G2. After 6 weeks, the plasma insulin levels of G4 increased by about 36% compared to those of G2, and the plasma C-peptide levels of G4 were lower by about 18%. than those of G3. The results of the oral glucose tolerance test (OGTT) showed that CL01 lessened the blood glucose levels of G4 by 15% compared to G2. It was concluded that CL01 stimulates the proliferation of beta cells and promotes insulin secretion and may thus have a potential in improving the hypoglycemic effects among the diabetic symptoms.
세리포리아 락세라타 균사체 배양물(CL01)의 혈당 강하 효과를 확인하기 위해 in-vitro 및 in-vivo 시험을 수행하였 다. CL01이 INS-1 세포에서 덱사메타손에 의한 세포 사멸 방지 효과를 나타냈으며 3T3-L1 세포에서는 당수송체인 GLUT4의 발현을 증가시켰다. 제 2형 당뇨 마우스를 4 그룹 (normal control (G1), negative control (G2), positive control (G3), CL01 250 mg/kg (G4))으로 나누어 6주간 매일 CL01 을 투여한 후 혈중 지표를 확인한 결과, CL01 섭취군은 체중, 사료 및 물 섭취량에서 음성대조군 대비 유의적인 차이를 보이지 않았다. 투여 5주 후 CL01 섭취군의 혈당이 음성대조군 대비 유의적인 감소를 보였으며 6주 경과 시 혈중 인슐린은 36% 증가하였고 혈중 C-peptide 농도는 18% 감소하였다. 경구 당 부하 시험 결과 CL01 섭취군의 혈당이 음성대조군 대비 15% 감소하였다. 이상의 결과를 통해 CL01이 베타 세포를 증식시켜 인슐린 분비를 촉진하고 혈 당을 낮추는 효과가 있음을 알 수 있다.
Alternative Title
Ceriporia lacerata 균사체 배양물의 혈당 강하 활성 평가
Keimyung Author(s)(Kor)
장병철
Publisher
School of Medicine
Citation
Eun Ji Shin et al. (2015). Hypoglycemic effects of submerged culture of Ceriporia lacerata mycelium. 한국식품저장유통학회지, 22(1), 145–153. doi: 10.11002/kjfp.2015.22.1.145
Type
Article
ISSN
1738-7248
DOI
10.11002/kjfp.2015.22.1.145
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/37137
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
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