배양된 혈관내피세포에서 uncoupling protein 2 유전자과발현이 세포내 산화스트레스 및 endothelin-1 mRNA 발현에 미치는영향

Abstract

Objective : It has been hypothesized that atherosclerosis is an inflammatory disease. Risk factors for atherosclerosis can include generation of reactive oxygen species (ROS) and this ROS increase inflammatory gene expression in endothelium by acting as secondary messenger. Methods : UCP2-containing adenovirus was transfectedinto HAECs. ETk-mRNA and NRkB binding activity was determined by Nothern blot and electrophoretic mobility shift assay(EMSA). Results : When the cells were treated with 450uM linoleic acid or 300 uM oleic acid, both ET-u mRNA expression and NFkB activity in HAEC were increased. Conclusion : Increased availability of fatty acid increased ORS production, NFkB activy, and ET-1 mRNA expression in vascular endothlum.