Effect of Total Ginseng Saponin on the Opioid Receptor Binding in Mouse Brain

Abstract

The modulatory effects of total ginseng saponin (TGS) on the 1, 6, and opioid receptor binding in morphine tolerance and dependence were examined in this study. The specific [$^{3}H$ 수식 이미지]DAGO ([D-$Ala^2$ 수식 이미지, N-$Mephe^4$ 수식 이미지, $Glyco^4$ 수식 이미지] enkephalin) binding was significantly increased in chronic morphine (10 mg/kg, i.p.) treated mouse striatum. The specific [$^{3}H$ 수식 이미지]DPDPE ([D-$Pen^2$ 수식 이미지, D-$Pen^5$ 수식 이미지] enkephalin) binding was ignificantly increased following morphine treatment in the mouse striatum and cortex. Also, an apparent decrease in the affinity of [$^{3}H$ 수식 이미지]DPN (diprenorphine) was observed after chronic morphine treatment in mouse striatum and cortex. 7GS produced a sleight increase of specific [$^{3}H$ 수식 이미지]DAGO, [$^{3}H$ 수식 이미지]DPDPE binding and a significant increase of specific [$^{3}H$ 수식 이미지]DPN binding in the mouse brain striatum. In cortex, TGS produced an inhibition of specific [$^{3}H$ 수식 이미지]DAGO and [$^{3}H$ 수식 이미지]DPDPE binding and increase of the specific [$^{3}H$ 수식 이미지]DPN binding. The prolonged administration of TGS (25, 50, 100, and 150 mg/kg, i.p., 3 wks) produced an inhibition of increased [$^{3}H$ 수식 이미지]DAGO specific binding following morphine without significant changes in the agonist binding to and receptors in mouse striatum and cortex. These contracted alterations in $\mu$ 수식 이미지, $\gamma$ 수식 이미지 and $\kappa$ 수식 이미지 opiate receptor binding were dependent in TGS dogs and brain sites.

키워드 Total ginsneg saponin; morphine; opioid receptor binding