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자궁경부암에 있어서 Cyclin 유전자의 발현

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Author(s)
조준형차순도이태성조치흠백원기서성일서민호
Keimyung Author(s)
Cha, Soon DoLee, Tae SungCho, Chi HeumBaek, Won KiSuh, Seong IlSuh, Min Ho
Department
Dept. of Obstetrics & Gynecology (산부인과학)
Dept. of Microbiology (미생물학)
Journal Title
Obstetrics & Gynecology Science
Issued Date
1997
Volume
40
Issue
11
Abstract
Human papillomavirus(HPV) infection is one of the common sexual transmitted infections in Korea, and evidence that HPV plays a central role in the development of cervical cancer and its precusors is growing. HPV is a small, double stranded, non-enveloped DNA virus that infe-cts epithelial cells, replicates within their nuclei, and causes hyperproliferative lesions. HPV ty-pe 16 and 18 are most strongly associated with high-grade lesion and cancer of the cervix. HPV DNA is integrated into the genome of the host cell in most invasive cancers. Integration usually results in disruption of the viral transcriptional regulatory circuit, which may faciliate cell proliferation by allowing deregulated expression of the viral oncogenes E6 and E7. The high risk HPV E6 protein can form complex with tumor suppressor protein p53 and faciliate the rapid degradation of p53 via the ubiquitin dependent proteolytic system. The high risk E7 protein bi-nds pRB related proteins and then displaces E2F like transcription factors from pRB related proteins. By inactivating p53 and pRB, HPV can induce proliferation of infected cells. In order to elucidate the association between HPV infection and the expression of cyclin genes in cervical cancers, we studied the prevalence of HPV infection and the expression level of cyclin genes(cyclin A, B, C, D1 and E) in nine cervical cancer samples. The presence of HPV infection was examined by RT-PCR and restriction enzyme analysis. HPV was detected in all cases(9/9);HPV type 16 was detected in 6 cases, HPV type 18 in 2 cases and an unknown type was detected in one case. The expression level of cyclin genes were evaluated by semiquantitative RT-PCR method. Expression of examined cyclin genes(cyclin A, B, C, D1 and E) were increased in all cancer tissues, and cyclin B and C expression were remarkably increased. There are no differences of expression levels of cyclins depending on infected HPV types.
Alternative Title
Expression of Cyclin Genes in Cervical Carcinomas
Keimyung Author(s)(Kor)
차순도
이태성
조치흠
백원기
서성일
서민호
Publisher
School of Medicine
Citation
조준형 et al. (1997). 자궁경부암에 있어서 Cyclin 유전자의 발현. Obstetrics & Gynecology Science, 40(11), 2457–2464.
Type
Article
ISSN
2287-8572
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/37466
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Obstetrics & Gynecology (산부인과학)
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