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갑상선세침흡인검사에 의한 갑상선 조직 및 말초혈액의 시토킨 발현과 말초혈액 단핵구의 인터페론 감마 생성에 관한 연구

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Author(s)
조성래박근용전영준송홍석권기영김윤년한승범이인규이규보이재태김보완
Keimyung Author(s)
Park, Keun YongJeon, Young JuneSong, Hong SukKwon, Ki YoungKim, Yoon NyunHan, Sung BeomLee, In Kyu
Department
Dept. of Internal Medicine (내과학)
Journal Title
대한내분비학회지
Issued Date
1995
Volume
10
Issue
1
Abstract
Cytokine production was studied in thyroid fine needle aspirates and peripheral blood and the production of interferon-gamma by peripheral blood mononuclear cell(PBMC) culture in response to interleukin-2(IL-2) stimulation was also studied from patients with hyperthyroidism, non toxic goiter, thyroid nodule. The expression of glycer aldehyde 3-phosphate dehydrogenase(GAPDH), interleukin-1beta(IL-1beta), IL-2, interleukin-8(IL-8), platelet- derived growth factor-A(PDGF-A) and interferon-gamma(IFN-gamma) chain was assessed by RT-PCR(reverse transcriptase polymerase chain reaction) in fine needle aspirates of thyroid and peripheral blood mononuclear cell : the samples were obtained from 7 patients with hyperthyroidism, 6 patients with non toxic goiter, 7 patients with thyroid nodule. A dose of IL-2(25 U/ml) was utilized to induce IFN-gamma production by PBMC from all patients.The results were as follows:1) In case of cytokine expression of fine-needle aspirates, GAPDH and IL-1beta, IL-8 were expressed highly but IFN-gamma, IL-2 were not expressed in hyperthyroidism and non-toxic goiter, thyroid nodule. PDGF-A was expressed in hyperthyroidism and thyroid nodule but not in non toxic goiter. 2) In case of cytokine expression of PBMC, GAPDH, IL-1beta were expressed in hyperthyroidism and non toxic goiter, thyroid nodule and highly expressed after IL-2 stimulation than before. But PDGF-A was more expressed in non toxic goiter and thyroid nodule than hyperthyroidism. Also, IFN-gamma was less expressed in thyroid nodule than hyperthyroidism and non toxic goiter. 3) The incremental increase in IFN-gamma value in supernatants of PBMC culture was significantly higher in patients with non toxic goiter than that in PBMC from hyperthyroidism and thyroid nodule(p<0.05).Therefore it seems that the cytokine production was found in hyperthyroidism and non toxic goiter and thyroid nodule. There were variability in their distribution each other, in general, higher expressed in hyperthyroidism than non toxic goiter. And RT-PCR Method that employed should be sufficiently sensitive to permit the analysis of cytokine gene expression in fine needle aspiration biopsies from patients with thyroid disease(J Kor Soc Endocrinol 10: 13-25, 1995)
Alternative Title
Analysis of Cytokine Gene Expression in Thyroid Aspirates and Peripheral Blood Mononuclear Cell and in vitro Production of Interferon-Gamma by Peripheral Blood Mononuclear Cell Culture
Keimyung Author(s)(Kor)
박근용
전영준
송홍석
권기영
김윤년
한승범
이인규
Publisher
School of Medicine
Citation
조성래 et al. (1995). 갑상선세침흡인검사에 의한 갑상선 조직 및 말초혈액의 시토킨 발현과 말초혈액 단핵구의 인터페론 감마 생성에 관한 연구. 대한내분비학회지, 10(1), 13–25.
Type
Article
ISSN
2093-596X
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/37715
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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