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폐쇄형 c-myb Antisense Oligonucleotides 개발과 암세포의 성장억제 연구

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Author(s)
문익재박종구
Keimyung Author(s)
Park, Jong Gu
Department
Dept. of Molecular Medicine(분자의학)
Institute for Medical Science (의과학연구소)
Journal Title
대한암학회지
Issued Date
1999
Volume
31
Issue
2
Abstract
Purpose: Aberrant expression of the c-myb gene is often detected in transformed leukemic cells. Inhibition of c-myb expression by antisense oligos could be an effective way to abort rapid growth of leukemic cells. Developing stable antisense oligos combined with enhanced delivery into cells would be of great use in developing an effective anti-cancer molecular agent.
Materials and Methods: Selection of target sites was carried out by employing computer simulation of mRNA secondary structures. Multiple antisense oligo sequences were adjoined and AS-oligos were then covalently closed to evade exonuclease activities. C-myb antisense oligos with a novel structure were complexed with cationic liposomes and used to treat HL-60 leukemic cells.
Results: We developed covalently closed antisense oligos which harbor four adjoined antisense sequences. The c-myb antisense oligos were found to be exceptionally stable and effective in specifically ablating c-myb mRNA. The antisense oligos were able to inhibit growth of leukemic cell line (HL-60) by about 80%. Antisense effect was more pronounced when the cells were treated twice with the antisense oligos at lower concentrations. Conclusions: The novel covalently closed antisense oligo (CMAS-oligos) was found to be effective and exceptionally stable. Growth of HL-60 was significantly inhibited, showing a rational way to develop an effective molecular anti-cancer agent.
Alternative Title
Development of Covalently Closed c-myb Antisense Oligonucleotides for Growth Inhibition of Leukemic Cells
Keimyung Author(s)(Kor)
박종구
Publisher
School of Medicine
Citation
문익재 and 박종구. (1999). 폐쇄형 c-myb Antisense Oligonucleotides 개발과 암세포의 성장억제 연구. 대한암학회지, 31(2), 348–359.
Type
Article
ISSN
0496-6872
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38077
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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