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염색체 미세절단법을 이용한 표지염색체의 전좌 부위에 대한 DNA Painting Probe 제작

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Author(s)
박찬상이인환최인장김대광
Keimyung Author(s)
Lee, In HwanChoi, In JangKim, Dae Kwang
Department
Dept. of Anatomy (해부학)
Dept. of Medical Genetics (의학유전학)
Institute for Cancer Research (암연구소)
Journal Title
대한암학회지
Issued Date
2000
Volume
32
Issue
3
Abstract
Purpose: Chromosome microdissection has been recommended as a technology to overcome the limited problems of conventional cytogenetic analysis and is a direct approach to isolate DNA from specific interesting region of chromosome. KUMA-1 cell line has a specific reserved chromosome abnormality during process from primary cancer culture to continuous cell line development, der(2)t(2;?)(qter;?). So molecular analysis for translocation region of der(2) may be helpful to understand pathogenesis of this primary cancer. The aim of this study was to develop painting probe for the translocation region for molecular study in future about translocation region of der(2) of KUMA-1 cell line.
Materials and Methods: KUMA-1 cell line was derived from a squamous cell carcinoma of urinary bladder. The translocation breakpoint region of der(2) appeared in KUMA-1 cell line was microdissected and dissected chromosome segments were amplified by PCR reaction. Fluorescent in situ hybridization was conducted on KUMA-1 metaphase cells with the probe generated from PCR product to confirm the construction of painting probe containing the translocation breakpoint of der(2).
Results: Painting probe was hybridized to the metaphase chromosome of KUMA-1 cell line and two fluorescent signals were mapped to the translocation forming chromosomal region of der(2).
Conclusion: It was possible to construct the painting probe for the translocation region of der (2) by chromosome microdissection.
Alternative Title
Construction of DNA Painting Probe for Translocation Region of a Marker Chromosome by Chromosome Microdissection
Keimyung Author(s)(Kor)
이인환
최인장
김대광
Publisher
School of Medicine
Citation
박찬상 et al. (2000). 염색체 미세절단법을 이용한 표지염색체의 전좌 부위에 대한 DNA Painting Probe 제작. 대한암학회지, 32(3), 605–611.
Type
Article
ISSN
0496-6872
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38095
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Anatomy (해부학)
1. School of Medicine (의과대학) > Dept. of Medical Genetics (의학유전학)
3. Research Institutues (연구소) > Institute for Cancer Research (암연구소)
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