Complete Nucleotide Sequence of Cytochrome P450 2E1 Expressed in the Rat Brain

Abstract

From the RT-PCR amplifications using mRNA templates isolated from Sprague-Dawley rat brains, we isolated a cDNA fragment of 1,524 bp which covered the full coding information of the rat brain CYP2E1. Its nucleotide sequence was identical to the previously reported rat liver CYP2E1 mRNA except for the difference of one base (A to C at the nucleotide position 73). This difference also altered the amino acid Lys to Gln. However, no insertion or deletion of nucleotide(s) which could alter the reading frame was found within the structure of this rat brain CYP2E1. This study should provide the molecular basis regarding the pathophysiological function of CYP2E1 in the brain.