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Modulation of GABAA Receptor by Protein Kinase C in Autonomic Major Pelvic Ganglion Neurons

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Author(s)
Yeun Jong ChoiSeung-Kyu ChaDae-Ran KimIn Deok Kong
Keimyung Author(s)
Kim, Na Hyun
Department
Dept. of Nursing (간호학)
Journal Title
대한의생명과학회지
Issued Date
2008
Volume
14
Issue
2
Abstract
γ-aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the central nervous system, and its actions are mediated by subtypes of GABA receptors named as GABAA, GABAB, and GABAC. GABAA, receptor consisting of α, β, γ and δ subunits is a heterooligomeric ligand-gated chloride channel. This study was performed to investigate regulation of GABAA receptor by protein kinase C (PKC). Ion currents were recorded using gramicidine-perforated patch and whole cell patch clamp. mRNA encoding the subunits of PKC expressed in major pelvic ganglion (MPG) neurons was detected by using RT-PCR. The GABA-induced inward current was increased by PKC activators and decreased by PKC inhibitors, respectively. These effects were not associated with intracellular Ca2+ and OAG (1-oleoyl-2-acetyl-sn-glycerol), a membrane permeable diacylglycerol (DAG) analogue. These results mean that the subfamily of PKC participating in activation of GABAA receptor would be an atypical PKC (aPKC). Among theses, ξ isoform of aPKC was detected by RT-PCR. Taking together, we suggest that excitable GABAA receptor in sympathetic MPG neuron seemed to be regulated by aPKC, particular in ξ isoform. The regulatory roles of PKC on excitatory GABAA receptors in sympathetic neurons of MPG may be an important factor to control the functional activity of various pelvic organs such as bowel movement, micturition and erection.
Keimyung Author(s)(Kor)
김나현
Publisher
College of Nursing
Citation
Yeun Jong Choi et al. (2008). Modulation of GABAA Receptor by Protein Kinase C in Autonomic Major Pelvic Ganglion Neurons. 대한의생명과학회지, 14(2), 69–76.
Type
Article
ISSN
1738-3226
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38314
Appears in Collections:
2. College of Nursing (간호대학) > Dept. of Nursing (간호학)
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