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Novel Trimeric Complex for Efficient Uptake Plasmid Vector into HepG2 Cells

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Author(s)
Jong-Hyuck JooJong-Gu Park
Keimyung Author(s)
Park, Jong Gu
Department
Dept. of Molecular Medicine(분자의학)
Journal Title
대한의생명과학회지
Issued Date
2003
Volume
9
Issue
2
Abstract
Viral and non-viral vectors have been used in the delivery of genetic materials into animal cells and tissues, with each approach having pros and cons. Non-viral vectors have many useful merits such as easy preparation, low immunity and size tolerance of a transgene when compared to those of viral vectors. Delivery specificity may be achieved by complex formation between receptor ligands and a non-viral vector. In the present study, non-viral vector systems are investigated in an effort to find a practical delivery means for gene therapy, Receptor-ligand interaction between transferrin-receptor and transferrin was utilized for efficient gene transfer into cancer cells. A plasmid vector, pcDNA3 (LacZ) was ligated with a small duplexed oligo fragment in which a Biotin-VN™ phosphoramidite was placed in the middle of the oligo. The plasmid vector labeled by biotin was then conjugated with biotin-labeled transferrin via streptavidin. This trimeric conjugates were delivered to a hepatoma cell line, HepG2. The delivery efficiency of the trimeric conjugate was 2-fold higher than that of cationic liposomes used for transfection of a plasmid vector. These results demonstrate that a plasmid vector can be efficiently transferred into cells by forming a trimeric complex of plasmid vector-linker-ligand.
Keimyung Author(s)(Kor)
박종구
Publisher
School of Medicine
Citation
Jong-Hyuck Joo and Jong-Gu Park. (2003). Novel Trimeric Complex for Efficient Uptake Plasmid Vector into HepG2 Cells. 대한의생명과학회지, 9(2), 67–74.
Type
Article
ISSN
1738-3226
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38315
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
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