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두경부암 환자의 객담으로부터 Common MAGE Primer를 이용한 MAGE A1-6 mRNA의 검출의 의의 - 예비 보고

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Author(s)
이강대손성환문효성이영수정 헌김주연이환호장희경전창호박종욱
Keimyung Author(s)
Park, Jong Wook
Department
Dept. of Immunology (면역학)
Journal Title
대한이비인후과학회지
Issued Date
2003
Volume
46
Issue
12
Abstract
Background and Objectives:We have reported that the expression of MAGE gene is specific to the mRNA level or protein level in head and neck cancer tissues. In this study, we investigated the applicability of MAGE gene to molecular diagnosis of head and neck cancer by detecting MAGE mRNA with common MAGE primers in sputa of patients. Materials and Method: Nested RT-PCR with MAGE common primers were designed by authors and were performed to detect MAGE 1, 2, 3, 4a, 4b, 5a, 5b, and 6 (MAGE A1-6) genes in sputa obtained from 22 cancer patients (17 squamous cell carcinoma of laryngopharynx, 2 lung metastsis after surgery for tongue and hypopharyngeal cancer, 2 neck metastasis from primary lung cancer, and 1 case of sarcoma of hypopharynx) and 40 normal persons. Results:Of 17 sputa from squamous cell carcinoma patients, expression of MAGE mRNA was positive in 13 (76.5%) cases. MAGE was detected in 72.7% (8/11) of laryngeal cancer, 50% (1/2) of base of tongue cancer, and 100% in hypopharyngeal (2/2) and tonsillar cancer. According to T stage, T1, T2, T3, T4 was positive in 50% (2/4), 100% (5/5), 66.6% (2/3), and 80% (4/5) respectively. In case of primary lung cancer, metastatic lung cancer, and hypopharyngeal sarcoma, all expressed MAGE mRNA. Among 22 cancer patients, 81.8% (18/22) were positive. MAGE expression was detected in 5% (2/40) of the normal control. Conclusion:Nested RT-PCR with common MAGE primers was helpful for assessing the presence of cancer cell in the sputa of the respiratory tract. This examination can be used as a tumor marker for the screening of laryngopharyngeal cancers and early detection of recurrent cancers or lung metastasis after treatment.
Alternative Title
Significance of MAGE A1-6 mRNA Expression in Sputa of the Head and Neck Cancer Patients - A Preliminary Report
Keimyung Author(s)(Kor)
박종욱
Publisher
School of Medicine
Citation
이강대 et al. (2003). 두경부암 환자의 객담으로부터 Common MAGE Primer를 이용한 MAGE A1-6 mRNA의 검출의 의의 - 예비 보고. 대한이비인후과학회지, 46(12), 1070–1076.
Type
Article
ISSN
1225-035X
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38347
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
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