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Detection of MAGE and SSX Gene Expressions by RT-nested PCR Using Common Primers in Head and Neck Cancer

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Author(s)
Dal Won SongSeung Jin ShinDong Eun KimSeung Gon JungJong Wook ParkKang Dae Lee
Keimyung Author(s)
Song, Dal WonKim, Dong EunPark, Jong Wook
Department
Dept. of Otorhinolaryngology (이비인후과학)
Dept. of Immunology (면역학)
Journal Title
Clinical and Experimental Otorhinolaryngology
Issued Date
2008
Volume
1
Issue
2
Abstract
Objectives. The melanoma antigen gene (MAGE) and synovial sarcoma on X chromosome (SSX) gene families are silent in most normal adult tissues, but are expressed in a variety of malignant lesions. Therefore, detection of MAGE and SSX transcription may be useful for the diagnosis of head and neck cancers. The aim of this study is to detect MAGE and SSX gene transcripts of head and neck cancers using the MAGE 1-6 assay and the SSX 1-9 assay.
Methods. The transcripts of MAGE 1-6 and SSX 1-9 genes were detected by the MAGE 1-6 assay and the SSX 1-9 assay respectively, in cancer cell lines, cancer tissue, and induced sputum specimens from head and neck cancer patients.
Results. The transcripts of MAGE 1-6 and SSX 1-9 genes were detected in 82.8% and 75.9% of head and neck cancer tissues (N=29) respectively, and 96.6% of cancer tissues expressed at least one of MAGE 1-6 or SSX 1-9 genes. In the induced sputum of head and neck cancer patients (N=18), the transcripts of MAGE 1-6 and SSX 1-9 genes were detected in 72.2% and 77.8%, respectively, and 94.4% of the sputum specimens were positive for either the MAGE 1-6 or the SSX 1-9 assay.
Conclusion. These results suggest that the combination of MAGE 1-6 and SSX 1-9 assays may be useful in the diagnosis of head and neck cancer.
Keimyung Author(s)(Kor)
송달원
김동은
박종욱
Publisher
School of Medicine
Citation
Dal Won Song et al. (2008). Detection of MAGE and SSX Gene Expressions by RT-nested PCR Using Common Primers in Head and Neck Cancer. Clinical and Experimental Otorhinolaryngology, 1(2), 97–102. doi: 10.3342/ceo.2008.1.2.97
Type
Article
ISSN
1976-8710
DOI
10.3342/ceo.2008.1.2.97
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38389
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학)
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