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아데노바이러스를 이용한 쥐의 재조합 IL-10 (Ad:ratIL-10)의 거부반응 억제효과

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Author(s)
조원현김형태박관규최영국박종구
Keimyung Author(s)
Cho, Won HyunKim, Hyoung TaePark, Kwan KyuPark, Jong Gu
Department
Dept. of Surgery (외과학)
Dept. of Pathology (병리학)
Institute for Medical Science (의과학연구소)
Dept. of Molecular Medicine(분자의학)
Journal Title
대한이식학회지
Issued Date
2000
Volume
14
Issue
1
Abstract
Purpose: IL-10, one of the potent Th2 cytokine, has strong anti-inflammatory reaction and immunosuppression action by stabilization of polarized Th2 cell gene expression and blocking of function of antigen presenting cells. The aims of this study were to investigate the immunosuppressive effect of recombinant adenovirus mediated rat IL-10 (Ad:ratIL-10) in mouse to rat skin graft. Methods: The transgene expression of the recombinant adenovirus was confirmed by X-gal staining of Ad:LacZ infected skin graft. The skin graft was done between mouse and rat after 1 hour infection of donated skin with Ad:ratIL-10, which was already been produced in our Institute for Medical Science (의과학연구소). Checked gene expression in grafted skin by in situ RT-PCR and systemic blood by ELISA on day 1, 3,5 and 7. Immunosuppressive effect of the Ad:ratIL-10 was evaluated by graft survival and compared with control group which was infected by saline. Results: Transgene expression of recombinant adenovirus was peak on third day of skin graft and became disappeared on day 5 and 7. The same expression was confirmed by in situ RT-PCR of Ad:ratIL-10 infected skin graft. The systemic blood level of infected Ad:ratIL-10 checked by ELISA was undetectable but their expression checked in culture cell line of HeLa cell was 275 ng/mL on day 5. Mean grafted skin survival was 6.0±0.7 days in Ad:ratIL-10 group but statistically indistinguishable to control group of infection with saline (5.6 ± 0.6 days, p>0.05). Conclusion: In summary, the Ad:ratIL-10 alone, infected in grafted skin, was peak on day 3 after graft but showed no immunosuppressive effect on mouse-to-rat skin graft.
아데노바이러스를 vector로 한 재조합 IL-10 (Ad:ratIL-10)이 동향성 이종이식에서 거부반응 억제효과를 갖는지 알아보기 위해 ICR-mouse의 피부를 절제하여 Ad:ratIL-10에 감염시킨 후 Lewis rat에 이식하여 생착정도롤 조사하였다. 감염된 바이러스의 발현은 10^9 pfu/mL 농도에서 60분 감염시 이식 3일째에 절정을 보였고, 이식 5일 및 7일째는 다시 소멸된 것을 알 수 있었다. 쥐의 IL-10을 배양세포주에 감염시켰을 때 발현정도는 감염 후 5일째에 275 ng/mL 정도였으나, 생체에서 감염시킨 피부를 이식한 후 측정한 혈중농도는 ELISA검사에서 이식 후 어느 날에도 측 정되지 않을 정도로 소량이어서 국소감염된 Ad:ratIL-10의 전신 효과는 없음올 보여 주었다. Ad:ratIL-10 감염군의 피부생착기간은 6.0±0.7일로 대조군의 5.6 ±0.6일보다 약간 길었으나 통계적 유의성은 없었다 (p>0.05).
Alternative Title
Effect of Adenovirus Mediated Rat Interleukin-10 (Ad:ratIL-10) Gene Transfer in Mouse-to-rat Skin Graft
Keimyung Author(s)(Kor)
조원현
김형태
박관규
박종구
Publisher
School of Medicine
Citation
조원현 et al. (2000). 아데노바이러스를 이용한 쥐의 재조합 IL-10 (Ad:ratIL-10)의 거부반응 억제효과. 대한이식학회지, 14(1), 31–39.
Type
Article
ISSN
1598-1711
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38429
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. School of Medicine (의과대학) > Dept. of Surgery (외과학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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