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후두암종에서 Ebstein-Barr 바이러스 DNA와 Bcl-2 단백의 검색

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Author(s)
이상숙박남조박준식
Keimyung Author(s)
Lee, Sang Sook
Department
Dept. of Pathology (병리학)
Journal Title
대한두경부종양학술지
Issued Date
2000
Volume
16
Issue
1
Abstract
Objectives: Epstein-Barr virus(EBV) is a B-lymphotrophic virus with a tumorigenic potential. EBV infection has been recognized as the main cause of nasopharyngeal carcinoma and Burkitt's lymphoma. Bcl-2 protein expression is known to be up-regulated by the EBV-latency associated antigen latent membrane protein(LMP). The aim of this study was to determine the incidence of EBV in squamous cell carcinomas of the larynx and the relationship between the presence of EBV and bcl-2 expression. Patients and Methods: From January 1994 to December 1977, 35 patients with primary squamous cell carcinoma of the larynx were studied. EBV genome DNA was surveyed by polymerase chain reaction(PCR) assay and then compared the results of in situ hybridization(ISH) for EBER1 using digoxigenin-tailed oligonucleotide probe. The expression of bcl-2 protein was studied by immunohistochemistry(IHC) using bcl-2 monoclonal antibody. Results: By PCR, EBV genome was detected in 22 of 35(62.9%) squamous cell carcinomas of the larynx. Nineteen of 35 cases(54.3%) showed a positive nuclear staining for EBER1 in tumor cells by ISH. Three cases showed positivity in inflammatory cells by ISH and one of them showed a positive staining of both tumor cells and inflammatory cells. Eighteen of 32 specimens(62.5%) were positive for bcl-2 protein. There was no significant correlations between the presence of EBV DNA and bcl-2 expression. Conclusions: It could be concluded that high incidence of EBV in the laryngeal cancer tissue may indicate a probable role of EBV in the development of laryngeal carcinoma.
Alternative Title
Detection of Ebstein-Barr Virus DNA and Bcl-2 Protein in Laryngeal Squamous Cell Carcinoma
Keimyung Author(s)(Kor)
이상숙
Publisher
School of Medicine
Citation
이상숙 et al. (2000). 후두암종에서 Ebstein-Barr 바이러스 DNA와 Bcl-2 단백의 검색. 대한두경부종양학술지, 16(1), 14–19.
Type
Article
ISSN
1229-5183
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38487
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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