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경부 결핵성 임파선염 환자에서 PCR-RELP를 이용한 결핵균의 검출 및 확인

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Author(s)
이상숙조영록전지민최용석손은주박남조박준식
Keimyung Author(s)
Lee, Sang Sook
Department
Dept. of Pathology (병리학)
Journal Title
대한두경부종양학술지
Issued Date
1996
Volume
12
Issue
2
Abstract
Tuberculous cervical lymphadenitis is still an important cause of neck mass in Korea. Tuberculosis is an important differential diagnosis in patients of cervical lymphadenopathy. Rapid and sensitive test for the diagnosis of tuberculosis is essential for the approapiate treatment. Up to now, conventional diagnostic methods for M. tuberculosis were acid-fast bacilli(AFB) stain and culture of M. tuberculosis. The direct microscopic examination of AFB by Ziehl-Neelsen stain is rapid, but often negative. The culture for M. tuberculosis is time-consuming, taking 4 to 8 weeks. Recently various methods to detect Mycobacterial DNA, including PCR and restriction fragment length polymorphism(RFLP) analysis have been reported. Here we represent a simple method for the confirmation of M. tuberculosis and exclusion of the other Mycobacterial species by RFLP analysis and silver staining of polyacrylamide gel electrophoresis after nested PCR for a repetitive DNA sequence(IS986) specific for M. tuberculosis from fresh or paraffin-embedded biopsy specimens. This result leads us to conclude that this method is simple, rapid and possibly applicable to confirm M. tuberculosis and rule out the other Mycobacteria species from the clinical specimens in the clinical laboratories.
Alternative Title
Detection and Identification of Mycobacterium Tuberculosis in Patients with Tuberculous Cervical Lymphadenitis by PCR-RFLP
Keimyung Author(s)(Kor)
이상숙
Publisher
School of Medicine
Citation
이상숙 et al. (1996). 경부 결핵성 임파선염 환자에서 PCR-RELP를 이용한 결핵균의 검출 및 확인. 대한두경부종양학술지, 12(2), 169–176.
Type
Article
ISSN
1229-5183
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38488
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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