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동물모델에서의 급성통풍발작 유발인자와 조직학적 변화

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Author(s)
박재호
Keimyung Author(s)
Park, Jae Ho
Department
Dept. of Internal Medicine (내과학)
Journal Title
대한류마티스학회지
Issued Date
1998
Volume
5
Issue
2
Abstract
Objective: To see whether monosodium urate(MSU) crystals can complex ferric iron in vivo, and to compare the histologic inflammatory reaction to MSU with or without iron in the rat animal model. Methods: MSU crystals were synthesized and tested to be able to complex iron in vitro. MSU crystals which were confirmed to be able to complex iron in vitro were encapsulated and put in diffusion chambers made with discs and filters. They were implanted in 6 rats. The chambers were retrieved 24 hours later, and the concentration of iron associated with the urate was measured by using inductively coupled plasma emission spectroscopy. In order to compare the histologic inflammatory reaction to MSU with or without iron in the rat animal model, one group with 25mg MSU crystals suspended in 5ml phosphate buffered saline(PBS) and the other with 2Smg MSU crystals in 5ml of 5mM ferric chloride PBS were injected into 6-day-old air pouch model in rats. Pouch tissue specimen were collected from 5 rats at 6, 24 and 48 hours in two groups. Various histologic findings were graded and compared by two blinded observers using a 0-3 point scale(normal, minor, moderate and severe abnormalities). Results: The MSU crystals picked up ferric iron in vivo(8.7±1.8μmoles/g). On histologic evaluation, there were more pouch wall inflammatory cells in MSU plus iron group than MSU group at 48 hours(p<0.015). Conclusions: In MSU crystal-induced acute gouty inflammation, MSU crystals were confirmed to complex with iron in vivo. Histologically, the complexation of iron with MSU crystals showed increased inflammatory reaction in vivo. Iron may increase inflammatory response in acute gouty inflammation.
Alternative Title
A Precipitating Factor of Acute Gouty Inflammation in an Animal Model and its Histologic Contribution
Keimyung Author(s)(Kor)
박재호
Publisher
School of Medicine
Citation
박재호. (1998). 동물모델에서의 급성통풍발작 유발인자와 조직학적 변화. 대한류마티스학회지, 5(2), 169–176.
Type
Article
ISSN
1226-8070
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38496
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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