계명대학교 의학도서관 Repository

면역전자현미경 검색에 의한 뉴우모시스티스 카리니 표면 항원 물질의 발현

Metadata Downloads
Author(s)
권건영조승제김상표박관규장은숙
Keimyung Author(s)
Kwon, Kun YoungKim, Sang PyoPark, Kwan KyuChang, Eun Sook
Department
Dept. of Pathology (병리학)
Institute for Medical Science (의과학연구소)
Journal Title
대한병리학회지
Issued Date
1998
Volume
32
Issue
6
Abstract
This study was carried out to investigate the morphologic characteristics and localization of antigenic molecules of Pneumocystis carinii in experimentally induced P. carinii pneumonia in rats. After six weeks of administration of low protein diet and dexamethasone, Sprague-Dawley rats were sacrificed to submit lungs or bronchoalveolar lavage for the study. Monoclonal (092, 900, 902, and 904) and polyclonal (SP-D) antibodies were used for immunohistochemistry and immunoelectron microscopy (ITEM and ISEM). Immunohistochemically P. carinii organisms were well identified as clusters or separated forms in the alveolar spaces being frequently attached to the alveolar walls. Immunoelectron microscopically the adherences of gold particles were observed on the surface of all stages of the P. carinii. Occasionally positive immunogold labeling was observed in the cytoplasm of the trophozoites and on the pellicle of the intracystic bodies within the cysts. The monoclonal antibodies 092, 900, 902, and 904 reacted mainly with pellicles of P. carinii, whereas SP-D labeled on the pellicles, intracystic bodies, cytoplasms of the alveolar macrophages, and free floated surfactant material in the alveolar spaces. The immunogold particles were observed more diffusely and intensely in the cysts than in the trophozoites. These results indicate that antigen is mainly localized on the pellicles, and accumulated during development from the trophozoite to the cyst stages.
Key Words: Pneumocystis carinii; Electron microscopy; Immunohistochemistry; Immunoelectron microscopy
Alternative Title
Expression of Antigenic Surface Molecules of Pneumocystis Carinii by Immunoelectron Microscopic Examination.
Keimyung Author(s)(Kor)
장은숙
권건영
김상표
박관규
Publisher
School of Medicine
Citation
권건영 et al. (1998). 면역전자현미경 검색에 의한 뉴우모시스티스 카리니 표면 항원 물질의 발현. 대한병리학회지, 32(6), 393–403.
Type
Article
ISSN
0379-1149
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38598
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
공개 및 라이선스
  • 공개 구분공개
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.