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Isoproterenol로 유발된 흰쥐 심장비대에서의 심근세포사

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Author(s)
김수경장은숙권지윤
Keimyung Author(s)
Chang, Eun SookKim, Soo Kyung
Department
Dept. of Pathology (병리학)
Dept. of Pharmacology (약리학)
Cardiovascular Research Institute (심혈관연구소)
Journal Title
대한병리학회지
Issued Date
2001
Volume
35
Issue
3
Abstract
Background : Although cardiac hypertrophy contributes to cardiac failure, the underlying mechanism has not yet been precisely determined. This study was planned in order to determine the pathogenesis of heart failure following cardiac hypertrophy induced by -adrenergic stimulation. Methods : The extent of cardiac hypertrophy was assessed after administrating isoproterenol (ISO, 5 mg/kg) intraperitoneally for 6 hours, 1, 3, 5, 7 and 10 days. The hematoxylin-eosin, Masson's trichrome and phosphotungstic acid hematoxylin stains along with immunohistochemical stainings for proliferating cell nuclear antigen and Ki-67 were performed in the paraffin-embedded left ventricle sections. Apoptosis was assessed by DNA laddering and terminal deoxynucleotidyl transferase TdT-mediated dUTP-biotin nick end labeling (TUNEL) assay. TUNEL positive myocytes and some nonmyocytes appeared in the subepicardium at 6 hours after ISO administration. The localization of these cells was shifted to the subendocardium within 24 hours, and the TUNEL positive cells were seen throughout the myocardium on the 5th day after ISO treatment. Necrotic myocyte death occurred on the 3rd day of ISO administration in the subendocardium, and initial pericellular fibrosis was followed and increased thereafter, with replacement fibrosis accompanied by further necrotic myocyte cell death. Conclusions : Our data showed that ISO treatment induced apoptotic
myocyte death and superimposed necrotic myocyte death with subsequent fibrosis. The
observed cardiac myocyte death may reflect myocardial dysfunction.
Key Words : Isoproterenol, Hypertrophy, Left ventricle, Apoptosis
Alternative Title
Cardiac Myocyte Cell Death in Isoproterenol-Induced Cardiac
Hypertrophy in Rats
Keimyung Author(s)(Kor)
장은숙
김수경
Publisher
School of Medicine
Citation
김수경 et al. (2001). Isoproterenol로 유발된 흰쥐 심장비대에서의 심근세포사. 대한병리학회지, 35(3), 189–195.
Type
Article
ISSN
0379-1149
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38601
Appears in Collections:
3. Research Institutues (연구소) > Cardiovascular Research Center (심혈관연구소)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. School of Medicine (의과대학) > Dept. of Pharmacology (약리학)
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