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Puromycin Aminonucleoside 신병증에서 사구체 음이온 부위의 전자현미경적 변화

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Author(s)
김현철최찬오김영호박관규
Keimyung Author(s)
Kim, Hyun ChulPark, Kwan Kyu
Department
Dept. of Internal Medicine (내과학)
Dept. of Pathology (병리학)
Journal Title
대한병리학회지
Issued Date
2000
Volume
34
Issue
1
Abstract
An ultrastructural study was done on puromycin aminonucleoside (PAN) nephropathy which was induced in a group of Sprague-Dawley rats by a single intraperitoneally injected dose. To study the ultrastructural alteration of glomerular anionic sites renal tissue was stained with polyethyleneimine (PEI) as a cationic probe. The PEI method seemed to selectively stain heparan sulfate proteoglycan in the basement membrane and has been widely used to evaluate the changes of the basement membrane in human diseases as well as in experimental work. The experimental rats developed proteinuria three days after the PAN injection. Electron microscopic studies of glomeruli showed the loss of epithelial foot processes, formation of cytoplasmic vacuoles, microvillous formation, and increased numbers of lysosomes in the cytoplasm of podocytes. The anionic sites on the basement membrane with foot process fusion were mostly indistinguishable from those seen in control rats, but focal areas of loss or disarray of anionic sites were noted. The anionic sites were not seen on the basement membrane where the overlying epithelium was detached. The results suggest that proteinuria in PAN nephrosis may be primarily due to a glomerular epithelial lesion, leading to focal disarray of anionic sites or focal defects in the epithelial covering of the basement membrane. The loss of anionic sites in the basement membrane may result partially from the foot process fusion, but mostly from the epithelial detachment.
Key Words: Puromycin aminonucleoside nephropathy; Polyethyleneimine; Anionic site; Proteinuria
Alternative Title
Ultrastructural Changes in Glomerular Anionic Sites in Puromycin Aminonucleoside Nephropathy.
Keimyung Author(s)(Kor)
김현철
박관규
Publisher
School of Medicine
Citation
김현철 et al. (2000). Puromycin Aminonucleoside 신병증에서 사구체 음이온 부위의 전자현미경적 변화. 대한병리학회지, 34(1), 56–67.
Type
Article
ISSN
0379-1149
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38602
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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