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Transforming Growth Factor-β1에 대한 항체 및 유전자 치료가 피부 반흔 형성에 미치는 영향

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Author(s)
김준형한기환안종덕이인규김은주황미열박관규
Keimyung Author(s)
Kim, Jun HyungHan, Ki HwanLee, In KyuPark, Kwan Kyu
Department
Dept. of Plastic Surgery (성형외과학)
Dept. of Internal Medicine (내과학)
Dept. of Pathology (병리학)
Journal Title
대한병리학회지
Issued Date
2001
Volume
35
Issue
5
Abstract
Background : Transforming growth factor (TGF)- has a large variety of biological functions, including the modulation of inflammation and the immune system, and is presumed to play important roles in repairing wounds and reducing scarring. The objective of this study is to examine the effects of TGF- 1 on healing wounds and reducing scarring. We have also analysed the ability of the hemagglutinating virus of Japan (HVJ) liposome mediated antisense oligodeoxynucleotides (ODNs) to specifically inhibit wound-induced expressions of TGF- 1 proteins and mRNA in the rat skin. Methods : Skin wounds were created on the backs of 80 anesthetized rats. The first group of wounds, as the controls, was unmanipulated. The second group of wounds, as positive controls or an excessive scarring model, was injected with TGF- 1 subcutaneously. The third group of wounds was injected with anti- TGF- 1 antibody subcutaneously. The fourth group of wounds was injected with HVJ liposome mediated antisense ODNs for TGF- 1 subcutaneously. The wounds of all groups were bisected and analysed histologically 5, 10, 15, 30, and 50 days after the wounds were made. Results : All control wounds (TGF- 1 or no injection) healed with scarring, whereas the wounds treated with the antibody or antisense ODNs healed with less scar formation compared to the control group. The wounds treated with the antibody or antisense ODNs had fewer macrophages, less collagen and fibronectin contents than the other wounds. Northern blotting and in situ hybridization analysis showed that wound sites treated with HVJ liposome mediated antisense ODNs for TGF- 1 exhibited decreased levels of TGF- 1 mRNA after injury. Conclusions : These findings suggest an important new approach to controlling scarring in normal wound healing, complementing the practice of adding exogenous growth factors to chronic wounds in the attempt to inhibit collagen deposition.
Key Words : Transforming growth factor beta, Liposomes, Wounds and injuries, Oligoribonucleotides, Antisense
Alternative Title
The Effect of Antibody and Gene Therapy for Transforming
Growth Factor-β1 on Scar Formation
Keimyung Author(s)(Kor)
김준형
한기환
이인규
박관규
Publisher
School of Medicine
Citation
김준형 et al. (2001). Transforming Growth Factor-β1에 대한 항체 및 유전자 치료가
피부 반흔 형성에 미치는 영향. 대한병리학회지, 35(5), 424–432.
Type
Article
ISSN
0379-1149
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38603
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. School of Medicine (의과대학) > Dept. of Plastic Surgery (성형외과학)
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