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측두엽간질에서 해마경화에 따른 칼슘결합단백 발현 및 치상회 태상섬유의 시납스 재구성

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Author(s)
김상표김승필조승제조영록전지민강유나권건영장은숙
Keimyung Author(s)
Kim, Sang PyoKang, Yu NaKwon, Kun YoungChang, Eun Sook
Department
Institute for Medical Science (의과학연구소)
Dept. of Pathology (병리학)
Journal Title
대한병리학회지
Issued Date
1998
Volume
32
Issue
5
Abstract
This study was designed to identify expression of calcium-binding proteins and synaptic reorganizations of dentate mossy fibers in hippocampal sclerosis of human temporal lobe epilepsy. Hippocampal neuronal density was quantitively analyzed in temporal lobe epilepsy group (n=50) to investigate the degree of hippocampal sclerosis and it was compared with that of autopsy control (n=3). To verify the distribution of calcium-binding proteins in neurons of epileptic hippocampi, the parvalbumin (PV)-immunoreactive and calbindin-D28K (CB)-immunoreactive neurons were quantitively analyzed in each area of Ammon's horn by immunohistochemical stain. Also, to clarify synaptic reorganizations of the dentate mossy fibers, a part of each hippocampus was examined under light microscopy and transmission electron microscopy using Timm sulphide silver method. In epileptic hippocampi, severity of hippocampal sclerosis (HS) was graded four, which consisted of 3 cases with no HS, 6 mild HS, 12 moderate HS, and 29 severe HS. The hippocampal neuronal loss was most prominent in CA1, followed by CA4 and CA2. Expression of calcium-binding proteins was more prevalent in CA2 of all groups. The proportion of PV-immunoreactive neurons in CA1 and CA4 significantly increased in the moderate and severe HS group, whereas the proportion of CB-immunoreactive neurons did not correlated with the severity of HS. Timm granules were noted in inner molecular supragranular layer of dentate gyrus of epileptic hippocampi and they tended to increase in proportion along with the severity of hippocampal sclerosis. Transmission electron microscopy showed that supragranular Timm granules corresponded to synaptic terminals of mossy fibers. These results suggest that parvalbumin appears to have more protective effect against neuronal loss and that mossy fiber synaptic reorganization seems to play a major role in pathogenesis of hippocampal sclerosis of human temporal lobe epilepsy.
Key Words: Temporal lobe epilepsy; Hippocampal sclerosis; Calcium-binding protein; Timm granule; Mossy fiber synaptic reorganization
Alternative Title
Synaptic Reorganization of Dentate Mossy Fibers and Expression of Calcium Binding Proteins in Hippocampal Sclerosis of Temporal Lobe Epilepsy.
Keimyung Author(s)(Kor)
김상표
강유나
권건영
장은숙
Publisher
School of Medicine
Citation
김상표 et al. (1998). 측두엽간질에서 해마경화에 따른 칼슘결합단백 발현 및 치상회 태상섬유의 시납스 재구성. 대한병리학회지, 32(5), 328–336.
Type
Article
ISSN
0379-1149
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38636
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
3. Research Institutues (연구소) > Institute for Medical Science (의과학연구소)
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