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B16 멜라닌 세포에서 해양소재 추출물의 멜라닌 생성 저해 효과

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Author(s)
이찬장정희안은미박찬익
Keimyung Author(s)
Jang, Jung Hee
Department
Dept. of Pharmacology (약리학)
Journal Title
대한본초학회지
Issued Date
2012
Volume
27
Issue
4
Abstract
Objectives : Under normal condition melanin protects the skin from extracellular stimuli including ultraviolet (UV)-induced oxidative skin damages, but excess production and accumulation of melanin can induce hyperpigmentation causing esthetic problems. Therefore, in this study we tried to search for natural skin whitening materials from marine natural resources.
Methods : Water and ethanol extracts of marine natural resources were prepared from Porphyra thalli (PT), Laminariae thallus (LT), Ostreae concha (OC), Sargassum thallus (ST), Undaria thallus (UT), Codium thalli (CT), Enteromorpha thalli (ET), Syngnathoides biaculeatus (SB), and Hippocampus coronatus (Hc). Their effects against UVB and α-melanocyte stimulating hormone (α-MSH)-induced melanogenesis were investigated based on melanin formation in B16 mouse melanoma cells. The mRNA and protein expression of enzymes involved in the melanogenic process were further examined by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis, respectively.
Results : Water extract of Ostreae concha (OCW/E) effectively inhibited UVB and α-MSH-induced melanin production in B16 melanocytes, which seemed to be mediated by inhibition of mRNA expression of tyrosinase and tyrosinase-related protein 1 (TRP-1). In another experiment, ethanol extracts from Porphyra thalli (PTE/E), Laminariae thallus (LTE/E), Sargassum thallus (STE/E), Undaria thallus (UTE/E), Codium thalli (CTE/E), Syngnathoides biaculeatus (SBE/E), and Hippocampus coronatus (HcE/E) significantly suppressed UVB and α-MSH-induced melanin formation. Furthermore, ethylacetate fraction isolated form LTE/E (LTE/EEt) decreased UVB and α-MSH-elevated extracellular melanin levels via inhibition of tyrosinase protein expression.
Conclutions : These results suggest that marine natural resources such as Porphyra thalli, Laminariae thallus, Ostreae concha, Sargassum thallus, Undaria thallus, Codium thalli, Syngnathoides biaculeatus and Hippocampus coronatus have anti-melanogenic effects, thereby exhibiting high potentials to be utilized as one of the ingredients for the development of new whitening functional cosmetics.
Key words : B16 melanocytes, marine natural resources, melanogenesis, ultraviolet B
Alternative Title
Inhibitory Effects of Marine Natural Products on Melanogenesis in B16 Melanoma Cells
Keimyung Author(s)(Kor)
장정희
Publisher
School of Medicine
Citation
이찬 et al. (2012). B16 멜라닌 세포에서 해양소재 추출물의 멜라닌 생성 저해 효과. 대한본초학회지, 27(4), 73–80. doi: 10.6116/kjh.2012.27.4.73
Type
Article
ISSN
1229-1765
DOI
10.6116/kjh.2012.27.4.73
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38669
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pharmacology (약리학)
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