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베타아밀로이드로 유도된 신경소교세포 사멸에 대한 루테올린의 보호효과 연구

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Author(s)
박규환장정희
Keimyung Author(s)
Jang, Jung Hee
Department
Dept. of Pharmacology (약리학)
Journal Title
대한본초학회지
Issued Date
2013
Volume
28
Issue
6
Abstract
Objectives : The purpose of this study is to investigate neuroprotective effects and molecular mechanisms of luteolin against β-amyloid (Aβ25-35)-induced oxidative cell death in BV-2 cells.
Methods : The protective effects of luteolin against Aβ25-35-induced cytotoxicity and apoptotic cell death were determined by MTT dye reduction assay and TUNEL staining, respectively. The apoptotic cell death was further analyzed by measuring mitochondrial transmembrane potential and expression of pro- and/or anti-apoptotic proteins. To elucidate the molecular mechanisms underlying the protective effects of luteolin, intracellular accumulation of reactive oxygen species, oxidative damages, and expression of antioxidant enzymes were examined.
Results : Luteolin pretreatment effectively attenuated Aβ25-35-induced apoptotic cell death indices such as DNA fragmentation, dissipation of mitochondrial transmembrane potential, increased Bax/Bcl-2 ratio, and activation of c-Jun N-terminal kinase and caspase-3 in BV-2 cells. Furthermore, Aβ25-35-induced intracellular formation of reactive oxygen species and subsequent oxidative damages such as lipid peroxidation and depletion of endogenous antioxidant glutathione were suppressed by luteolin treatment. The neuroprotective effects of luteolin might be mediated by up-regulation of cellular antioxidant defense system via up-regulation of γ-glutamylcysteine ligase, a rate-limiting enzyme in the glutathione biosynthesis and superoxide dismutase, an enzyme involved in dismutation of superoxide anion into oxygen and hydrogen peroxide.
Conclusions : These findings suggest that luteolin has a potential to protect against Aβ25-35-induced neuronal cell death and damages thereby exhibiting therapeutic utilization for the prevention and/or treatment of Alzheimer’s disease.
Key words : luteolin, neuroprotection, β-amyloid, microglia, oxidative damages, antioxidant defense, Alzheimer’s disease
Alternative Title
Protective Effect of Luteolin against β-Amyloid-induced Cell Death and Damage in BV-2 Microglial Cells
Keimyung Author(s)(Kor)
장정희
Publisher
School of Medicine
Citation
박규환 and 장정희. (2013). 베타아밀로이드로 유도된 신경소교세포 사멸에 대한 루테올린의 보호효과 연구. 대한본초학회지, 28(6), 79–86.
Type
Article
ISSN
1229-1765
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38671
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Pharmacology (약리학)
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