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골반신경절 부교감신경세포에서 5-hydroxytriptamine3 유발 세포 내 칼슘농도 증가에 대한 Adenylyl Cyclase 및 Protein Kinase A의 조절

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Author(s)
김대란차승규박규상송은섭박영호송윤섭공인덕
Keimyung Author(s)
Kim, Na Hyun
Department
Dept. of Nursing (간호학)
Journal Title
대한비뇨기과학회지
Issued Date
2006
Volume
47
Issue
5
Abstract
Purpose: Serotonin has effects on the bladder contraction or urethral sphincter tone. Different subtypes of 5-hydroxytriptamine (5-HT) receptors appear to mediate the effects of serotonin on voiding. 5-HT1 and 5-HT2, metamorphic receptors, are examined well. However 5-HT3, ionotrophic receptors, are not examined well. Pelvic ganglia provide the majority of the innervation of the lower urinary tract. Major pelvic ganglia (MPG) in rats are autonomic ganglia, containing both sympathetic and parasympathetic neurons related with voiding. We examined the modulatory role of adenylyl cyclase (AC) and protein kinase A (PKA) in 5-HT3 induced intra cellular calcium increase in rat MPG.
Materials and Methods: The regulatory effects by AC and PKA were investigated in a single neuron of male rat major pelvic ganglia using patch clamp and fluorescence Ca2+ measurement techniques.
Results: Inward currents were induced by 5-HT (10μM) at only parasympathetic neurons of MPG. MDL7222 (10-6M), selective 5-HT3 receptor antagonists, completely abolished the 5-HT induced inward currents. 5-HT (10μM) induced intracelluar increases of calcium. These increases were blocked by an AC inhibitor SQ22536 (2x10-5M) and myristoylated PKA inhibitor (10-7M). Furthermore, foskolin (10-6M), AC activator, augmented the 5-HT induced intracellular calcium increase.
Conclusions: The activation of AC/PKA-dependent pathway could enhance 5-HT3 induced intacellular calcium increase in parasympathetic neurons of rat MPG and these is helpful for the better understanding the mechanisms of the bladder contraction or urethral sphincter tone. (Korean J Urol 2006;47:541-547)
Key Words: Calcium, Parasympathetic ganglia
Alternative Title
Modulatory Role of Adenylyl Cyclase and Protein Kinase A (PKA) in 5-hydroxytriptamine3 Induced Intracellular Calcium Increase in Parasympathetic Neurons of Rat Major Pelvic Ganglia
Keimyung Author(s)(Kor)
김나현
Publisher
School of Medicine
Citation
김대란 et al. (2006). 골반신경절 부교감신경세포에서 5-hydroxytriptamine3 유발 세포 내 칼슘농도 증가에 대한 Adenylyl Cyclase 및 Protein Kinase A의 조절. 대한비뇨기과학회지, 47(5), 541–547.
Type
Article
ISSN
2005-6737
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38714
Appears in Collections:
2. College of Nursing (간호대학) > Dept. of Nursing (간호학)
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