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신세포암 세포주에서 Interferon-gamma (IFN-γ)에 의한 Fas-매개성 세포사의 유도

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Affiliated Author(s)
권택규박종욱이성준서민호서성일백원기
Alternative Author(s)
Kwon, Taeg KyuPark, Jong WookLee, Sung JoonSuh, Min HoSuh, Seong IlBaek, Won Ki
Journal Title
대한비뇨기과학회지
ISSN
2005-6737
Issued Date
2000
Abstract
Purpose: Interferon-gamma (IFN-g) is a cytokine involved in cellular immune responses and has antiproliferative and cytotoxic effects on tumor cells in vitro. IFN-g is also capable of increasing the expression of MHC class I and class II molecules and tumor markers thereby enhancing the immune responses against the tumor cells. It has been reported in a number of studies that several cytokines including interleukin-2, IFN-a, IFN-g, and tumor necrosis factor-alpha, induce Fas antigen expression on tumor cell surfaces and make the tumor cells sensitive to Fas/Fas ligand (Fas/FasL)-mediated apoptosis. In the purpose of determining the effect of IFN-g on the Fas/FasL-Mediated apoptotic pathway in Renca cell line, inducibility of Fas antigen and anti-Fas Ab-mediated apoptosis were investigated. Materials and Methods: Fas mRNA transcript and Fas antigen expression were demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) and immunocytochemistry after IFN-g treatment on Renca. In order to induce cell death in the IFN-g-pretreated cells, Jo2, a cytotoxic anti-Fas Ab was used. The apoptotic characteristics of the Jo2-Induced Renca cell death were demonstrated by DNA fragmentation assay and FACS analysis. Results: Renca cells treated for 24 hours with 300U/ml of IFN-g, showed high-level expression of Fas mRNA and protein in RT-PCR and immunocytochemical staining. Jo2 was able to induce cell death after pretreatment of Renca with 150U/ml of IFN-g for 24 hours. Assays for DNA fragmentation revealed apoptosis-associated nucleosomal ladders in IFN-g and Jo2-treated Renca. Jo2-induced apoptosis was also confirmed by increased subdiploid DNA contents in flow cytometric analysis. Conclusions: These results suggest that IFN-g, by enhancing surface Fas expression, sensitizes Renca tumor cell line to Fas/FasL-mediated apoptosis.
Key word : IFN-g, Apoptosis, Fas antigen
Alternative Title
Fas-induced Apoptosis in Renal Cell Carcinoma Cell Line by Interferon-gamma (IFN-γ) Treatment
Department
Dept. of Immunology (면역학)
Dept. of Urology (비뇨의학)
Dept. of Microbiology (미생물학)
Publisher
School of Medicine
Citation
윤수정 et al. (2000). 신세포암 세포주에서 Interferon-gamma (IFN-γ)에 의한 Fas-매개성 세포사의 유도. 대한비뇨기과학회지, 41(5), 594–601.
Type
Article
ISSN
2005-6737
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/38759
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Urology (비뇨의학)
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