자궁내막에서 Endothelin 의 조직면역화학

Abstract

The endometrium is a target tissue for the actions of ovarian estradiol and progesterone, which are responsible for it`s growth and differentiation in preparation for implantation. Recently peptide endothelin may be a paracrine factor in the endometrium. Our objective was to determine the localization of immunoreactive endothelin in human cyclic endometrium and gynecologic disease. Fixed dated endometrial tissue(n=42) and tissues of adenomyosis (n=16), adenomatous hyperplasia (n=11), cystic hyperplasia (n=12), endometral cancer(n=19), endometrioma(n=10). Intensity of positive staining was given a score of 0 (negative), 1(low), 2(moderate) or 3 (strongly positive) for each group within each section. Comparison of immunohistochemistry with endothelin from different group (proliferative, secretory, menstrual, senile phase) were conducted with Kruskal Wallis one way analysis. Comparison of each section(adenomyosis, hyperplasis, endometrial cancer, endometrioma) with luminal epithelium were conducted with Bonferroni-adjusted Mann Whitney test. Positive immunostaining for endothelin was observed in all endometrial tissue. Low level of immunoreactivity were observed in endometrial stroma in all tissues. The strongest staining was seen in luminal epithelium throughout the secretory phase. And moderate staining of luminal epithelium in adenomyosis, hyperlasia, endometrial cancer and endometrioma were showed. Key word : Endothelin; Menstrual cycle, Adenomyosis, Hyperplasia.