신경상피 뇌종양의 관련 유전자 연구

Abstract

Cancer may be a disease of genes, arising from genetic damage of diverse sorts-recessive and dominant mutations, large rearrangement of DNA and gene translocation on chromosomes, all leading to distorsions of either the expression or biochemical function of genes. The search for these genetic damage in neoplastic cells now is the most important in cancer research. It has been found that the cancer relevant genes were located on the specific regions of chromosomes. To determine whether epidermal p w t h factor receptor(EGFR), PS3 and bcr genes located in chromosomes 7, 17 and 22 are altered, we examined 12 neuroepithelial tumor with Southern blot analydfive low grade astrocytoma, two high grade astrocytoma, two medulloblastoma, one oligodendroglioma, one ependymoma, one choroid plexus papilloma). The loss of heterozygosity(L0H) of EGFR gene was detected in two cases of medulloblastoma. The rearrangement of EGFR gene was detected in a case of ependymoma The LOH of Pa gene was found in a case of choroid plexus papilloma and low grade astrocytoma The rearrangement of P53 gene was found in a case of oligodendroglioma. The M H of bcr gene was observed in two cases of medulloblastoma and low grade astrocytoma The rearrangement of bcr gene was observed in two cam of high grade astrocytoma. These results suggested that tumorigenesis and tumor development in the neuroepithelial tumor may involve specific gene changes in chromosomes 7, 17 and 22.

신경상피 뇌종양 12례(양성 성상세포종 5례, 악성 성상세포종 2례, 수아세포종 1례, 핍지신경교종 1례, 상의세포종 1례, 맥락총 유두종 1례)를 대상으로 염색체 7, 17 및 22번에 각각 좌위한 EGRR, P^(53) 및 bcr 유전자의 변화를 찾기위해 Southern blot 분석을 시행하였다. 결과에서 EGFR 유전자의 이형접합체 소실은 2례의 수아세포종 그리고 재배열은 1례의 상의세포종에서 검색되었고, P^(53) 유전자의 이형접합체 소실은 1례의 맥락총 유두종과 1례의 양성 성상세포종에서, 재배열은 1례의 핍지신경교종에서 발견되었으며 bcr 유전자의 이형접합체 소실은 2례의 수아세포종과 2례의 양성 성상세포종에서 그리고 재배열은 2례의 악성 성상세포증에서 관찰되었다. 위의 결과로 신경상피 뇌종양의 암화과정이나 발달에 염색체 7, 17 및 22번내의 특이한 유전자 변화가 관여하는 것으로 사료되었다.