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일차 배양 해마신경세포에서 NMDA- 및 Glutamate- 유도전류의 특성

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Author(s)
김일만손은익김동원김인홍임만빈송대규박원균배재훈최하영Il-Man KimEun-Ik SonDong-Won KimIn-Hong KimMan-Bin YimDae-Kyu SongWon-Kyun ParkJae-Hun BaeHa-Young Choi
Keimyung Author(s)
Kim, ElSon, Eun IkKim, Dong WonKim, In HongYim, Man BinSong, Dae KyuBae, Jae HoonPark, Won Kyun
Department
Dept. of Neurosurgery (신경외과학)
Dept. of Physiology (생리학)
Dept. of Medical Education (의학교육학)
Journal Title
대한신경외과학회지
Issued Date
2000
Volume
29
Issue
11
Abstract
Objectives:This study was performed in cultured rat hippocampal neurons to investigate the acute electrophysiological features of ionotropic glutamate receptors which act as a major excitatory neurotransmitter in mammalian brain.
Method:Glutamate receptor agonists were applied into the bath solution embedding in whole-cell patch-clamp recording of single hippocampal neuron.
Results:In voltage-clamped at -60mV and the presence of 1mmol Mg2+, extracellulary applied NMDA did not induce any inward current. Both the elimination of Mg2+ and addition of glycine in bath, however, elicited a NMDAinduced inward current. Mg2+ block current was increased gradually in more negative potentials from –30mV, showing a negative slope in I-V plot with Mg2+. Glutamate-induced current represented an outward rectification. A non-NMDA receptor component occupied about 40% of glutamate-induced current in the voltage range of –80mV to +60mV.
Conclusion:Present study suggests that glutamate activates acutely the non-NMDA receptors which induces an inward current in the level of resting membrane potential. This makes the membrane potential increase and can activate the NMDA receptors that permit calcium influx against Mg2+ block. At the depolarized state of neuron, there may be recovery mechanisms of membrane potential to repolarize irrespective of voltage-dependent potassium
channels in the hippocampal neurons.
Alternative Title
Characteristics of NMDA- and Glutamate-Induced Currents in Primary Cultured Rat Hippocampal Neurons
Keimyung Author(s)(Kor)
김엘
손은익
김동원
김인홍
임만빈
송대규
배재훈
박원균
Publisher
School of Medicine
Citation
김일만 et al. (2000). 일차 배양 해마신경세포에서 NMDA- 및 Glutamate- 유도전류의 특성. 대한신경외과학회지, 29(11), 1429–1436.
Type
Article
ISSN
1225-8245
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39148
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Medical Education (의학교육학)
1. School of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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