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I형 콜라겐지지체의 다양한 가교처리 방법이 연골막성 연골재생에 미치는 영향

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Author(s)
손대구임중재손경희양은경Dae Gu SonJoong Jae LimKyounghee SohnEun Kyung Yang
Keimyung Author(s)
Son, Dae Gu
Department
Dept. of Plastic Surgery (성형외과학)
Journal Title
대한성형외과학회지
Issued Date
2006
Volume
33
Issue
6
Abstract
PURPOSE: Collagen is the principal structural biomolecule in cartilage extracellular matrix, which makes it a logical target for cartilage engineering. In this study, porous type I collagen scaffolds were cross-linked using dehydrothermal(DHT) treatment and/or 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide(EDC), in the presence and absence of chondroitin-6-sulfate(CS) for cartilage regeneration. METHODS: Cartilage defects were created in the proximal part of the ear of New Zealand rabbits. Four types of scaffolds(n=4) were inserted. The types included DHT cross-linked(Group 1), DHT and EDC cross- linked(Group 2), CS added DHT cross-linked(Group 3), and CS added DHT and EDC cross-linked(Group 4). Histomorphometric analysis and cartilage-specific gene expression of the reconstructed tissues were evaluated respectively 4, 8, and 12 weeks after implantation. RESULTS: The largest quantity of regenerated cartilage was found in DHT cross-linked groups 1 and 3 in the 8th week and then decreased in the 12th week, while calcification increased. Calcification was observed from the 8th week and the area increased in the 12th week. Group 4 was treated with EDC cross-linking and CS, and the matrix did not degrade in the 12th week. Cartilage-specific type II collagen mRNA expression increased with time in all groups. CONCLUSION: CS did not increase chondrogenesis in all groups. EDC cross-linking may prevent chondrocyte infiltration from the perichondrium into the collagen scaffold.
Alternative Title
The Effect of Various Methods of Cross-linking in Type I Collagen Scaffold on Cartilage Regeneration
Keimyung Author(s)(Kor)
손대구
Publisher
School of Medicine
Citation
손대구 et al. (2006). I형 콜라겐지지체의 다양한 가교처리 방법이 연골막성 연골재생에 미치는 영향. 대한성형외과학회지, 33(6), 723–731.
Type
Article
ISSN
1015-6402
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39266
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Plastic Surgery (성형외과학)
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