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반흔형성 과정에서 Sp1 전사인자 조절에 의한 TGF-β1 및 CTGF의 발현

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Author(s)
박동만손대구한기환이선영채영미장영채박관규Dong Man ParkDae Gu SohnKi Hwan HanSun Young LeeYoung Mi ChaeYoung Chae ChangKwan Kyu Park
Keimyung Author(s)
Son, Dae GuHan, Ki Hwan
Department
Dept. of Plastic Surgery (성형외과학)
Journal Title
대한성형외과학회지
Issued Date
2006
Volume
33
Issue
1
Abstract
This study is to examine the relationship between TGF-b1 expression and CTGF expression, and to evaluate the effect of Sp1 blockade on the expression of TGF-b1, CTGF and extracellular genes, clones of fibroblasts stably transfected with Sp1 decoy ODN. R-Sp1 decoy ODN was highly resistant to degradation by nucleases or serum, compared to the linear or phosphorothioated-Sp1 decoy ODN. Skin wounds were created on the back of 36 anesthetized rats. They were divided into four groups-the rats with normal skin, with wounded skin without decoy, with wounded skin injected with R-Sp1 decoy, and with wounded skin injected with mismatched R-Sp1 decoy, respectively. Skins were collected at 3rd, 5th, 7th, 14th day after wounding. Cellular RNA was extracted by RT-PCR analysis. TGF-beta1 and CTGF were deeply related with skin fibrosis during scar formation and it appeared that TGF-beta1 may cause the induction of CTGF expression. R-Sp1 decoy ODN inhibited TGF-beta1 and CTGF expression both in cultured fibroblasts and in the skin of rats. These results indicate that targeting Sp1 with R-type decoy efficiently blocks extracellular matrix gene expression, and suggest an important new therapeutic approach to control the scarring in normal wound healing and fibrotic disorders.
Alternative Title
The Effect of the Transcriptional Regulation of Sp1 for TGF-β1 and CTGF Expression in Scar Formation
Keimyung Author(s)(Kor)
손대구
한기환
Publisher
School of Medicine
Citation
박동만 et al. (2006). 반흔형성 과정에서 Sp1 전사인자 조절에 의한 TGF-β1 및 CTGF의 발현. 대한성형외과학회지, 33(1), 39–45.
Type
Article
ISSN
1015-6402
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39270
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Plastic Surgery (성형외과학)
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