1년 이상 냉동 보관한 흡인 지방조직 내의 세포 생존
- Author(s)
- 손대구; 오재훈; 최태현; 김준형; 한기환; Daegu Son; Jaehoon Oh; Taehyun Choi; Junhyung Kim; Kihwan Han
- Keimyung Author(s)
- Son, Dae Gu; Choi, Tae Hyun; Kim, Jun Hyung; Han, Ki Hwan
- Department
- Dept. of Plastic Surgery (성형외과학)
- Journal Title
- 대한성형외과학회지
- Issued Date
- 2009
- Volume
- 36
- Issue
- 2
- Abstract
- PURPOSE: The use of an autogenous fat graft has become a common procedure in plastic surgery. However, questions remain concerning on the viability of fat cells and preservation method of aspirated fat. The purpose of this study is to examine the viability of fat cells stored at -20degrees C in the freezer for 1 year after harvest from abdominal liposuction. METHODS: Eighteen adults(aged from 24 to 65 years, 16 female and 2 male) were selected for this study. Harvested aspirated fat tissues were obtained by suction-assisted lipectomy and frozen at -20degrees C commercial refrigerator for one year(average 12.5 months). The viability of fat cells in specimens were measured after thawing. The numbers of viable cells were measured on a fluorescence microscope after staining with fluorescein diacetate and propidium iodide. GPDH(Glycerol-3-phosphate dehydrogenase) activity was measured. Cell culture was done for 3 weeks. RESULTS: There were no viable cells under the fluorescence microscope, no detectable GPDH activity, and no cultured cells. CONCLUSION: These findings suggest that aspirated fat after frozen storage for one year at -20degrees C freezer is inadequate to reuse.
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