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1년 이상 냉동 보관한 흡인 지방조직 내의 세포 생존

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Author(s)
손대구오재훈최태현김준형한기환Daegu SonJaehoon OhTaehyun ChoiJunhyung KimKihwan Han
Keimyung Author(s)
Son, Dae GuChoi, Tae HyunKim, Jun HyungHan, Ki Hwan
Department
Dept. of Plastic Surgery (성형외과학)
Journal Title
대한성형외과학회지
Issued Date
2009
Volume
36
Issue
2
Abstract
PURPOSE: The use of an autogenous fat graft has become a common procedure in plastic surgery. However, questions remain concerning on the viability of fat cells and preservation method of aspirated fat. The purpose of this study is to examine the viability of fat cells stored at -20degrees C in the freezer for 1 year after harvest from abdominal liposuction. METHODS: Eighteen adults(aged from 24 to 65 years, 16 female and 2 male) were selected for this study. Harvested aspirated fat tissues were obtained by suction-assisted lipectomy and frozen at -20degrees C commercial refrigerator for one year(average 12.5 months). The viability of fat cells in specimens were measured after thawing. The numbers of viable cells were measured on a fluorescence microscope after staining with fluorescein diacetate and propidium iodide. GPDH(Glycerol-3-phosphate dehydrogenase) activity was measured. Cell culture was done for 3 weeks. RESULTS: There were no viable cells under the fluorescence microscope, no detectable GPDH activity, and no cultured cells. CONCLUSION: These findings suggest that aspirated fat after frozen storage for one year at -20degrees C freezer is inadequate to reuse.
Alternative Title
Viability of Cells in Aspirated Fat Tissue after 1 Year Cryopreservation
Keimyung Author(s)(Kor)
손대구
최태현
김준형
한기환
Publisher
School of Medicine
Citation
손대구 et al. (2009). 1년 이상 냉동 보관한 흡인 지방조직 내의 세포 생존. 대한성형외과학회지, 36(2), 135–139.
Type
Article
ISSN
1015-6402
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39331
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Plastic Surgery (성형외과학)
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