계명대학교 의학도서관 Repository

아래쪽배벽동맥피판의 생존향상을 위한 VEGF와 COMP-angiopoietin-1 유전자 치료

Metadata Downloads
Author(s)
유은경손대구김형태이인규최태현김준형한기환Eun Kyung YooDaegu SonHyung Tae KimIn Kyu LeeTaehyun ChoiJunhyung KimKihwan Han
Keimyung Author(s)
Son, Dae GuChoi, Tae HyunKim, Jun HyungHan, Ki HwanKim, Hyoung Tae
Department
Dept. of Plastic Surgery (성형외과학)
Dept. of Surgery (외과학)
Journal Title
대한성형외과학회지
Issued Date
2009
Volume
36
Issue
1
Abstract
PURPOSE: Partial necrosis of skin flaps remains a substantial problem in reconstructive surgery. We investigated the potential use of an adenovirus vector encoding the VEGF, COMP-angiopoietin-1 gene in an attempt to promote the viability of the inferior epigastric artery flap in a rat model. METHODS: Three by six cm lower abdominal transverse skin flaps, supplied only by the left inferior epigastric artery, were designed. After skin flap elevation, the adenovirus VEGF and adenovirus COMP-angiopoietin-1 were injected into the distal portion of the flap, which has a high tendency of developing flap ischemia. Control animals were injected with the same volume of normal saline. On 3, 7 and 14 days after the flap elevation, the flap survival and vascularization were assessed using Visitrak digital(R), CD31 immunohistochemistry in addition to evaluating the general histological characteristics. RESULTS: There was a significant increase in the mean percentage of flap viability by 89.8%, 91.1% and 94.8% in flaps transfected with adenovirus VEGF, COMP- angiopoietin-1, coadministraion of VEGF and COMP- angiopoietin-1 at seven days, and by 95.6%, 94.8% and 96.3% at 14 days. Histological assessment revealed that there were more blood vessels formed after adenovirus with VEGF, COMP-angiopoietin-1 or VEGF plus COMP- angiopoietin-1 than with adenovirus Lac Z. CONCLUSION: The results of this study suggest that adenovirus-mediated VEGF, COMP-angiopoietin-1 gene therapy, promote therapeutic angiogenesis in patients that undergo reconstructive procedures.
Alternative Title
Improvement of the Inferior Epigastric Artery Flap Viability Using Adenovirus-mediated VEGF and COMP-angiopoietin-1
Keimyung Author(s)(Kor)
손대구
최태현
김준형
한기환
김형태
Publisher
School of Medicine
Citation
유은경 et al. (2009). 아래쪽배벽동맥피판의 생존향상을 위한 VEGF와 COMP-angiopoietin-1 유전자 치료. 대한성형외과학회지, 36(1), 1~10-1~10.
Type
Article
ISSN
1015-6402
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39333
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Plastic Surgery (성형외과학)
1. School of Medicine (의과대학) > Dept. of Surgery (외과학)
Authorize & License
  • Authorize공개
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.