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Pulsed-Field Gel Electrophoresis를 이용한 Methicillin-Resistant Staphylococcus aureus의 분자역학적 분석

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Author(s)
김영재전동석김재룡
Keimyung Author(s)
Jeon, Dong SeokKim, Jae Ryong
Department
Dept. of Laboratory Medicine (진단검사의학)
Journal Title
대한임상병리학회지
Issued Date
2001
Volume
21
Issue
2
Keyword
MRSADNA fingerprintingPFGEMolecular epidemiological analysisAntimicrobial sensitivity test
Abstract
Background : Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen that
is frequently involved in outbreaks of nosocomial infections. We did moleculoepidemiological analysis
of MRSA strains isolated at the Dongsan Medical Center from January 1998 to June 1998 by
means of a pulsed-field gel electrophoresis (PFGE) and antimicrobial resistance phenotyping.
Methods : One hundred six strains of MRSA, isolated from patients, were analyzed and classified
by DNA fingerprinting with PFGE by CHEF-DR III (Bio-Rad Co., USA) after digestion with SmaI. An
antimicrobial sensitivity test was performed and the patterns were compared to the PFGE patterns.
Results : Among the 106 isolates, 28 different PFGE patterns were identified. Nineteen patterns
were responsible for more than one strain (2 to 24 strains). Twenty-seven patterns showed over 65%
in the similarity coefficient and were classified into 8 groups (I-VIII). Group II strains were mostly isolated
from wound and sputum, whereas group III from pleural fluid and urine. Most of group II isolates
were from patients of the wards (40% of NS isolates, 71% of OS isolates and 60% of IM isolates)
and ICUs (50% of SICU and MICU isolates). The antibiogram separated the isolates into 11
types. Type 6 was predominant and subdivided into groups II, III and VII of the PFGE patterns.
Conclusions : Molecular chromosomal DNA fingerprinting with PFGE revealed the clonal relationships
of some MRSA isolates at the Dongsan Medical Center. Thus, molecular epidemiological analysis
with PFGE would be valuable for delineating epidemic isolates of MRSA, for identifying the
nosocomial reservoirs, and for tracing their intrahospital spread.
Alternative Title
Molecular Epidemiologic Analysis of Methicillin-Resistant Staphylococcus aureus using Pulsed-Field Gel Electrophoresis
Keimyung Author(s)(Kor)
전동석
김재룡
Publisher
School of Medicine
Citation
김영재 et al. (2001). Pulsed-Field Gel Electrophoresis를 이용한 Methicillin-Resistant Staphylococcus aureus의 분자역학적 분석. 대한임상병리학회지, 21(2), 122–128.
Type
Article
ISSN
1015-6445
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39422
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
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